NucleoMag HMW DNA

NucleoMag HMW DNA enables magnetic bead-based isolation of high-quality, high-molecular-weight (HMW) DNA for long-range sequencing. The kit is compatible with a wide range of sample materials, including cells, human and animal tissue, plant tissue, whole blood, saliva, swabs, bacteria, and yeast. The workflow can be automated on liquid handling platforms in combination with NucleoMag SEP and on magnetic rod systems such as the IsoPure Mini.

Cat. #	Product	Size	Price	License	Quantity	Details
744160.1	NucleoMag® HMW DNA	96 Preps	USD $414.00
The NucleoMag HMW DNA kit is designed for the isolation of HMW DNA (from 50–200 kb) from a wide range of sample materials. Following lysis and clarification of the sample material, NucleoMag B-Beads are used for DNA isolation. After washing steps, highly pure DNA is eluted from the beads. The NucleoMag HMW DNA kit can be used either manually or automated on standard liquid handling instruments or automated magnetic separators. Reagents and magnetic beads necessary for isolation of 96 samples are included; however, additional reagents may be required for lysis, depending on your sample type. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back NucleoMag HMW DNA successfully isolates high-molecular-weight DNA from a wide variety of sample types. NucleoMag HMW DNA successfully isolates high-molecular-weight (HMW) DNA from a wide variety of sample types. Genomic DNA from a variety of sample materials was isolated using NucleoMag HMW DNA and samples were analyzed for fragment size distribution using an Agilent Bioanalyzer 2100. Ladder fragment sizes are bp lengths. Isolated DNA fragments obtained using NucleoMag HMW were over 50 kb in length for all sample materials tested. Back DNA isolated with NucleoMag HMW DNA demonstrates higher yield and purity compared to a competitor kit. DNA isolated with NucleoMag HMW DNA demonstrates higher yield and purity compared to a competitor kit. HMW DNA was purified from a variety of sample materials with NucleoMag HMW DNA or with Company Z's HMW DNA kit. A NanoDrop 2000 was used to assess DNA yields (Panel A) and purity as determined by the A_260/280 (B) and A_260/230 (C) values. Back 744160.1: NucleoMag HMW DNA

Cat. #

Product

Size

Price

License

Quantity

Details

744160.1

NucleoMag® HMW DNA

96 Preps

USD $414.00

The NucleoMag HMW DNA kit is designed for the isolation of HMW DNA (from 50–200 kb) from a wide range of sample materials. Following lysis and clarification of the sample material, NucleoMag B-Beads are used for DNA isolation. After washing steps, highly pure DNA is eluted from the beads.

The NucleoMag HMW DNA kit can be used either manually or automated on standard liquid handling instruments or automated magnetic separators. Reagents and magnetic beads necessary for isolation of 96 samples are included; however, additional reagents may be required for lysis, depending on your sample type.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Overview

Isolate HMW DNA (up to 200 kb) via magnetic bead-based technology
Obtain high DNA yield and purity from a wide range of sample materials
Process samples on an automated platform or manually

More Information

Format	Magnetic beads
Fragment Size	From 50–200 kb
Typical yield	Depends on sample type and amount
Sample materials	Animal tissue, bacteria, blood, cells, human tissue, plant, saliva, yeast
Sample amounts for select sample materials	≤1 x 10⁶ cultured cells ≤20 mg human/animal tissue ≤50 mg plant leaves ≤25 mg gram-negative bacteria ≤35 mg gram-positive bacteria ≤50 mg yeast
Automated platform compatibility	Compatible with most open robotic platforms. Please contact technical support for more information.
Elution volume	100–200 µl
Processing time	60–120 min, depending on manual or automated processing

Applications

Isolation of high-molecular-weight DNA from mammalian tissues, blood samples, invertebrates, plants, bacteria, and yeast
Typical downstream applications: long-read sequencing (e.g., SMRT, Nanopore, Next GEM)

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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NucleoMag HMW DNA

Notice to purchaser