Isolation of genomic DNA from plants using magnetic beads—NucleoMag Plant
NucleoMag Plant is designed for the rapid parallel purification of genomic DNA from plant tissues using magnetic beads. After lysis of the sample, genomic DNA is selectively bound to the NucleoMag beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation), the risk of cross-contamination is greatly reduced. NucleoMag technology can be easily adapted to common laboratory automation platforms. It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.
Overview
- One-tube procedure minimizes risk of cross-contamination
- Small elution volumes (>50 µl) are possible
- Yield does not depend on elution volume
- Easily adapted to automated use
More Information
| Technology | Magnetic bead technology |
| Format | Highly reactive superparamagnetic beads (size of beads: 1–3 µm) |
| Processing | Manual or automated |
| Starting material | 20–50 mg plant tissue |
| Fragment size | 300 bp–>30 kb |
| Typical yield | 10–20 µg |
| A260/280 | >1.6–1.9 |
| Elution volume | >50 µl |
| Preparation time | <120 min/96 preps (manual use) |
| Binding capacity | 0.4 µg/1 µl beads |
Applications
- Manual or automated isolation of DNA from plant tissue
- Isolated DNA can be used for PCR, Southern blotting, any kind of enzymatic reaction
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Greater yield and purity than plant DNA extraction kits from leading competitors
In this tech note we compare the yield and purity of genomic DNA recovered using NucleoSpin Plant II to DNA extracted using leading competitors. Data from a variety of difficult plant tissue samples are shown.
Read the tech note Plant RNA kitsTakara Bio USA, Inc.
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