Magnetic bead-based isolation of genomic DNA from forensic samples—NucleoMag DNA Forensic
The Macherey-Nagel DNA Forensic series is designed to match the requirements of forensic laboratories. Use the magnetic bead-based NucleoMag DNA Forensic kit for manual or automated DNA isolation in a high-throughput (HTP) format or the spin column-based NucleoSpin DNA Forensic kit for manual DNA isolation from individual samples. The uniform buffer chemistry of the spin column- and magnetic bead-based technologies enables these two formats to provide comparable, consistently high levels of gDNA recovery, allowing for maximum flexibility in choosing the optimal kit based on your purification technology preference.
The Macherey-Nagel DNA Forensic series is designed to match the requirements of forensic laboratories. Use the magnetic bead-based NucleoMag DNA Forensic kit for manual or automated DNA isolation in a high-throughput (HTP) format or the spin column-based NucleoSpin DNA Forensic kit for manual DNA isolation from individual samples. The uniform buffer chemistry of the spin column- and magnetic bead-based technologies enables these two formats to provide comparable, consistently high levels of gDNA recovery, allowing for maximum flexibility in choosing the optimal kit based on your purification technology preference.
The NucleoMag Forensic kit allows manual or automated purification of DNA using proven NucleoMag magnetic bead technology. The kit is designed for forensic samples with low or high DNA content. A specialized lysis buffer in the kit enables the isolation of DNA from forensic samples, contact traces, or buccal swabs. Inhibitors are completely removed from all forensic samples, allowing positive and reliable STR profiles to be generated from these samples. If sample carriers such as buccal swabs are used, it is necessary to remove the carrier after incubation with the lysis buffer. NucleoSpin Forensic Filters are recommended for efficient and convenient carrier removal. The filters allow a long-term incubation of lysis buffer and sample, followed by an easy and fast separation using centrifugation.
The NucleoMag product family is based on magnetic bead technology. Nucleic acids are bound to the magnetic beads under chaotropic salt conditions, contaminants are washed away with ethanolic wash buffers, and pure DNA can be eluted from the beads using elution buffer. The NucleoMag technology can be easily adapted to common laboratory automation platforms. It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations. For more information on adapting any NucleoMag product to your workstation, please contact your local distributor or MN directly.
Overview
- Excellent DNA purity from all forensic samples
- Uniform buffer system for high-throughput analysis (NucleoMag DNA Forensic) and for single sample (NucleoSpin DNA Forensic) analysis
- Conformity to ISO 18385 for reliable DNA profiling
More Information
| Technology | Magnetic bead technology |
| Format | Highly reactive superparamagnetic beads |
| Processing | Manual or automated |
| Starting material | Forensic samples, contact traces (e.g., dried blood spots, cigarette filters, swabs) |
| Typical yield | e.g., 1–3 µg from buccal swabs |
| Typical concentration | 1 ng/µl |
| Elution volume | 25–50 µl |
| Binding capacity | 0.4 µg/µl beads |
Applications
- Manual or automated isolation of DNA from forensic samples
- STR analysis, qPCR, any kind of enzymatic reaction
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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