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Genomic DNA purification kits

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Magnetic bead-based isolation of genomic DNA from swabs—NucleoMag DNA Swab

Isolate gDNA from cotton and synthetic swabs using automation-friendly magnetic beads.

The NucleoMag DNA Swab kit enables manual or automated purification of genomic DNA from swab specimens using proven NucleoMag magnetic bead technology. NucleoMag DNA Swab can isolate human and/or microbial gDNA from cotton swabs and synthetic swabs (e.g., flocked swabs). It is designed for isolation of gDNA from common epithelial surfaces, including dental, nasopharyngeal, and vaginal swabs as well as other samples including cigarette filters and dried blood spots. After sample lysis with Proteinase K, gDNA is purified from 300 µl of sample. The entire NucleoMag DNA Swab protocol can be automated on common liquid handling platforms.

The NucleoMag DNA Swab kit enables manual or automated purification of genomic DNA from swab specimens using proven NucleoMag magnetic bead technology. NucleoMag DNA Swab can isolate human and/or microbial gDNA from cotton swabs and synthetic swabs (e.g., flocked swabs). It is designed for isolation of gDNA from common epithelial surfaces, including dental, nasopharyngeal, and vaginal swabs as well as other samples, such as cigarette filters and dried blood spots. After sample lysis with Proteinase K, gDNA is purified from 300 µl of sample. The entire NucleoMag DNA Swab protocol can be automated on common liquid handling platforms.

To simplify the separation of swab material from lysate, the kit can be combined with NucleoSpin Forensic Filters or the NucleoSpin Trace Filter Plate (please note that the NucleoSpin Trace Filter Plate requires additional material for operating under vacuum or centrifugation). The NucleoMag DNA Swab kit can be used either manually or on automated liquid handling instruments (such as Hamilton, Tecan and Eppendorf platforms) and magnetic separators (such as the KingFisher Duo and Flex).

The NucleoMag product family is based on magnetic bead technology. Nucleic acids are bound to the magnetic beads under chaotropic salt conditions, contaminants are washed away with ethanolic wash buffers, and pure DNA can be eluted from the beads using elution buffer. NucleoMag technology can be easily adapted to common laboratory automation platforms. It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.

Over thirty human pathogens have been isolated from swabs with the NucleoMag DNA Swab kit and successfully detected by qPCR, including:

SpeciesPathogen typeCt value
Acetinobacter baumanii Gram negative bacteria 23.05
Atopobium vaginae Gram positive bacteria 19.92
Bacteriodes fragilis Gram negative bacteria 21.66
Candida albicans Fungal 27.78
Candida dubliniensis Fungal 24.87
Candida glabrata Fungal 25.89
Candida krusei Fungal 17.76
Candida lusitaniae Fungal 20.41
Candida parapsilosis Fungal 26.94
Candida tropicalis Fungal 20.19
Chlamydia trachomatis Gram negative bacteria 24.31, 23.90
Citrobacter freundii Gram negative bacteria 22.78
Enterococcus faecalis Gram positive bacteria 22.27
Escherichia coli Gram negative bacteria 26.05
Gardnerella vaginalis Gram negative and gram positive bacteria 16.79
Herpes simplex type 1 dsDNA virus 27.92, 27.44
Herpes simplex type 2 dsDNA virus 21.60, 21.49
Klebsiella oxytoca Gram negative bacteria 26.86
Lactobacillus jensenii Gram positive bacteria 15.30
Mobiluncus mulieris Gram negative and gram positive bacteria 27.75
Morganella morganii Gram negative bacteria 23.14
Mycoplasma genitalium Mycobacteria 15.33, 15.17
Mycoplasma hominis Mycobacteria 22.72
Neisseria gonorrhoeae Gram negative bacteria 14.93, 14.91
Prevotella bivia Gram negative bacteria 24.48
Proteus mirabilis Gram negative bacteria 22.81
Proteus vulgaris Gram negative bacteria 23.10
Providencia stuartii Gram negative bacteria 22.90
Pseudomonas aeruginosa Gram negative bacteria 18.56
Serratia marcescens Gram negative bacteria 24.13
Staphylococcus aureus Gram positive bacteria 22.50
Staphylococcus saprophyticus Gram positive bacteria 24.63
Streptococcus agalactiae Gram positive bacteria 23.06
Trichomonas vaginalis Protozoan 24.84, 25.20

Note: An additional pre-digestion step with Lysis Buffer FLB and Liquid Proteinase K significantly improves the lysis of some pathogens—particularly yeast—and may be required for positive results.

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Cat. # Product Size Price License Quantity Details
744601.1 NucleoMag® DNA Swab 1 x 96 Preps USD $380.00

The NucleoMag DNA Swab kit is designed for high-throughput purification of genomic DNA from various swab specimens, enabling efficient sample processing for a wide array of genetic testing applications. The kit has been validated with both cotton and synthetic swabs (e.g., flocked swabs), and employs a magnetic bead-based purification technology that can be readily automated on common liquid handling instruments (e.g., Hamilton, Tecan, and Eppendorf platforms) or magnetic separators such as the KingFisher Duo or Flex. Following reversible adsorption of nucleic acids to superparamagnetic beads, highly pure DNA is eluted under low-salt conditions and can be incorporated directly in downstream applications such as qPCR or NGS. The NucleoMag DNA Swab kit typically yields 1–3 µg of DNA per prep, and enables processing of 96 samples in ~30 min on the KingFisher Flex (excluding sample lysis).

Cat. # 744601.1 includes sufficient quantities of reagents and materials for processing 1 x 96 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data
744601.4 NucleoMag® DNA Swab 4 x 96 Preps USD $1265.00

The NucleoMag DNA Swab kit is designed for high-throughput purification of genomic DNA from various swab specimens, enabling efficient sample processing for a wide array of genetic testing applications. The kit has been validated with both cotton and synthetic swabs (e.g., flocked swabs), and employs a magnetic bead-based purification technology that can be readily automated on common liquid handling instruments (e.g., Hamilton, Tecan, and Eppendorf platforms) or magnetic separators such as the KingFisher Duo or Flex. Following reversible adsorption of nucleic acids to superparamagnetic beads, highly pure DNA is eluted under low-salt conditions and can be incorporated directly in downstream applications such as qPCR or NGS. The NucleoMag DNA Swab kit typically yields 1–3 µg of DNA per prep, and enables processing of 96 samples in ~30 min on the KingFisher Flex (excluding sample lysis).

Cat. # 744601.4 includes sufficient quantities of reagents and materials for processing 4 x 96 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data
744601.24 NucleoMag® DNA Swab 24 x 96 Preps USD $5855.00

The NucleoMag DNA Swab kit is designed for high-throughput purification of genomic DNA from various swab specimens, enabling efficient sample processing for a wide array of genetic testing applications. The kit has been validated with both cotton and synthetic swabs (e.g., flocked swabs), and employs a magnetic bead-based purification technology that can be readily automated on common liquid handling instruments (e.g., Hamilton, Tecan, and Eppendorf platforms) or magnetic separators such as the KingFisher Duo or Flex. Following reversible adsorption of nucleic acids to superparamagnetic beads, highly pure DNA is eluted under low-salt conditions and can be incorporated directly in downstream applications such as qPCR or NGS. The NucleoMag DNA Swab kit typically yields 1–3 µg of DNA per prep, and enables processing of 96 samples in ~30 min on the KingFisher Flex (excluding sample lysis).

Cat. # 744601.24 includes sufficient quantities of reagents and materials for processing 24 x 96 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

automated nucleic acid purification Automate your workflow
Strips, plates and blocks for high-throughput nucleic acid purification HTP accessories
Isolate circulating DNA from plasma NucleoMag cfDNA

Overview

  • High-throughput DNA isolation for genetic testing 
  • Developed for cotton as well as synthetic swabs 
  • Small elution volumes—as low as 50 µl
  • Suitable for manual or automated processing

More Information

Technology Magnetic bead technology
Format Highly reactive superparamagnetic beads
Processing Manual or automated
Starting material Swabs (cotton or synthetic)
Sample amount 300 µl reconstituted swab lysate
Fragment size >300 bp–approximately 50 kb (depending on sample processing)
Typical yield 1–3 µg DNA (depending on sample amount and quality)
Typical concentration 10–30 ng/µl
Typical A260/280 1.6–1.9
Elution volume 50–100 µl
Preparation time 120 min/96 preps (manual preparation) 
30 min/96 preps (automated on KingFisher Flex; excluding lysis)
Theoretical binding capacity 0.4 μg/μl beads

Applications

  • High-throughput genetic testing
  • Manual or automated isolation of DNA from cotton or synthetic swabs (e.g., COPAN FLOQSwabs and Puritan HydraFlock swabs)
  • Typical downstream applications: qPCR, NGS, STR analysis, enzymatic reactions

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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