NucleoMag cfDNA

The NucleoMag cfDNA kit is designed for rapid manual or automated isolation of circulating cell-free DNA (cfDNA) from 1–10 ml of human plasma obtained using EDTA or Cell-Free DNA BCT collection tubes. The kit enables efficient recovery of fragmented DNA ≥50 bp in size via reversible adsorption of nucleic acids to paramagnetic beads under optimized buffer conditions. cfDNA can be eluted in 50–200 µl of buffer and is ready for applications such as qPCR, ddPCR, NGS, etc.

The protocol provided in the NucleoMag cfDNA user manual is intended for processing 2-ml inputs of blood plasma, but kit reagents are provided in sufficient quantities for processing input volumes ranging from 1–10 ml. In comparison with a competitor kit developed for cfDNA purification from plasma, NucleoMag cfDNA was shown to provide higher, more consistent yields from challenging samples containing low amounts of cfDNA.

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Cat. #	Product	Size	Price
744550.1	NucleoMag® cfDNA	48 Preps	USD $431.00
The NucleoMag cfDNA kit provides an efficient, robust method for high-throughput isolation of circulating cell-free DNA (cfDNA) from 1–10 ml of human plasma obtained using EDTA or Cell-Free DNA BCT collection tubes. The kit enables recovery of fragmented DNA ≥50 bp in size via reversible adsorption of nucleic acids to paramagnetic beads under optimized buffer conditions, and eluted cfDNA is ready for downstream applications such as qPCR, ddPCR, NGS, etc. NucleoMag cfDNA is compatible with manual or automated processing and enables preparation of 24 samples in less than an hour (excluding cell lysis) on the KingFisher Flex platform. Cat. # 744550.1 includes sufficient quantities of reagents and materials for processing 48 samples. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back The NucleoMag cfDNA procedure The NucleoMag cfDNA procedure. Sample lysis is achieved by incubation of plasma with Liquid Proteinase K and Lysis Buffer MCF1 at 56°C. Buffer MCF2 and paramagnetic NucleoMag P-Beads are then added, enabling optimal binding of nucleic acids. Three washing steps efficiently remove contaminating substances, such as PCR inhibitors, after magnetic separation. Residual ethanol is removed by a drying step, and highly pure DNA is eluted from the paramagnetic beads with 50–200 µl of Elution Buffer MCF5—a slightly alkaline buffer of low ionic strength (5 mM Tris/HCl, pH 8.5). The NucleoMag cfDNA kit can be used either manually or on automated liquid handling platforms or magnetic separators. Back Reliable purification of cfDNA from different volumes of plasma Reliable purification of cfDNA from different volumes of plasma. The NucleoMag cfDNA (formerly known as NucleoMag DNA Plasma) kit was used to isolate cfDNA from 2–10 ml inputs of EDTA-treated human plasma. Analysis of purified cfDNA on the Agilent Bioanalyzer 2100 system using the High Sensitivity DNA Kit yielded a characteristic peak at 170 bp for each input volume. The total yield from each sample was determined using the PicoGreen quantification assay with the Quant-iT PicoGreen kit on a Synergy HT Multi-Detection Microplate Reader (Biotek). The consistent correlation observed between cfDNA yield and input volume demonstrates the reliable performance of the NucleoMag cfDNA kit. Back Efficient cfDNA recovery regardless of input volume Efficient cfDNA recovery regardless of input volume. cfDNA isolated from varying input amounts of EDTA-treated human plasma using the NucleoMag cfDNA kit yielded clear bands upon measurement by capillary electrophoresis with the Agilent Bioanalyzer 2100 system and the High Sensitivity DNA Kit. Even for volumes as low as 2 ml, purified cfDNA was clearly visible. Back Higher, more consistent yields relative to a competitor kit Higher, more consistent yields relative to a competitor kit. Total cfDNA was purified from 2-ml inputs of EDTA-treated human plasma from four challenging donor samples containing low levels of cfDNA (<10 ng cfDNA/ml of plasma) using NucleoMag cfDNA (formerly known as NucleoMag DNA Plasma) and a kit from Competitor T. Total DNA recovery was quantified using the Qubit dsDNA High Sensitivity kit on a Qubit fluorometer. Relative to the kit from Competitor T, NucleoMag cfDNA provided higher yields with lower sample-to-sample variability.
744550.4	NucleoMag® cfDNA	4 x 48 Preps	USD $1559.00
The NucleoMag cfDNA kit provides an efficient, robust method for high-throughput isolation of circulating cell-free DNA (cfDNA) from 1–10 ml of human plasma obtained using methods such as EDTA or Cell-Free DNA BCT collection tubes. The kit enables recovery of fragmented DNA ≥50 bp in size via reversible adsorption of nucleic acids to paramagnetic beads under optimized buffer conditions. The eluted cfDNA is ready for downstream applications such as qPCR, ddPCR, NGS, etc. NucleoMag cfDNA is compatible with manual or automated processing, and enables preparation of 24 samples in less than an hour (excluding cell lysis) on the KingFisher Flex platform. Cat. # 744550.4 includes sufficient quantities of reagents and materials for processing 192 samples. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back The NucleoMag cfDNA procedure The NucleoMag cfDNA procedure. Sample lysis is achieved by incubation of plasma with Liquid Proteinase K and Lysis Buffer MCF1 at 56°C. Buffer MCF2 and paramagnetic NucleoMag P-Beads are then added, enabling optimal binding of nucleic acids. Three washing steps efficiently remove contaminating substances, such as PCR inhibitors, after magnetic separation. Residual ethanol is removed by a drying step, and highly pure DNA is eluted from the paramagnetic beads with 50–200 µl of Elution Buffer MCF5—a slightly alkaline buffer of low ionic strength (5 mM Tris/HCl, pH 8.5). The NucleoMag cfDNA kit can be used either manually or on automated liquid handling platforms or magnetic separators. Back Reliable purification of cfDNA from different volumes of plasma Reliable purification of cfDNA from different volumes of plasma. The NucleoMag cfDNA (formerly known as NucleoMag DNA Plasma) kit was used to isolate cfDNA from 2–10 ml inputs of EDTA-treated human plasma. Analysis of purified cfDNA on the Agilent Bioanalyzer 2100 system using the High Sensitivity DNA Kit yielded a characteristic peak at 170 bp for each input volume. The total yield from each sample was determined using the PicoGreen quantification assay with the Quant-iT PicoGreen kit on a Synergy HT Multi-Detection Microplate Reader (Biotek). The consistent correlation observed between cfDNA yield and input volume demonstrates the reliable performance of the NucleoMag cfDNA kit. Back Efficient cfDNA recovery regardless of input volume Efficient cfDNA recovery regardless of input volume. cfDNA isolated from varying input amounts of EDTA-treated human plasma using the NucleoMag cfDNA kit yielded clear bands upon measurement by capillary electrophoresis with the Agilent Bioanalyzer 2100 system and the High Sensitivity DNA Kit. Even for volumes as low as 2 ml, purified cfDNA was clearly visible. Back Higher, more consistent yields relative to a competitor kit Higher, more consistent yields relative to a competitor kit. Total cfDNA was purified from 2-ml inputs of EDTA-treated human plasma from four challenging donor samples containing low levels of cfDNA (<10 ng cfDNA/ml of plasma) using NucleoMag cfDNA (formerly known as NucleoMag DNA Plasma) and a kit from Competitor T. Total DNA recovery was quantified using the Qubit dsDNA High Sensitivity kit on a Qubit fluorometer. Relative to the kit from Competitor T, NucleoMag cfDNA provided higher yields with lower sample-to-sample variability.

Overview

Consistent cfDNA recovery from 1–10 ml plasma samples
Efficient purification of fragmented DNA as small as 50 bp
No PCR inhibition regardless of the sample volume processed

More Information

	NucleoMag cfDNA
Technology	Magnetic bead technology
Format	Highly reactive superparamagnetic beads
Processing	Manual or automated
Starting material	1–10 ml human EDTA/Cell-Free DNA BCT plasma
Fragment size	50 bp
Typical yield	Depending on sample source, storage, and quality
A_260/280	1.6–1.9
Elution volume	50–200 µl
Preparation time	55 min/24 preps (2 ml; excl. lysis)*
Binding capacity	0.3 µg/µl beads

*Established on a KingFisher Flex.

Applications

Manual or automated isolation of circulating cell-free DNA from human plasma obtained using EDTA or Cell-Free DNA BCT collection tubes
Typical downstream applications: NGS, qPCR, ddPCR

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Cell-free DNA-seq library prep in under 2 hours

ThruPLEX Plasma-Seq Kit

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Three-step, single-tube protocol can be completed in less than 2 hours
UDIs or dual index options for multiplexing up to 384 samples

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NucleoMag cfDNA

Notice to purchaser

The NucleoMag cfDNA procedure

Reliable purification of cfDNA from different volumes of plasma

Efficient cfDNA recovery regardless of input volume

Higher, more consistent yields relative to a competitor kit