NucleoSpin cfDNA Midi

NucleoSpin cfDNA Midi is designed for rapid preparation of highly pure cell-free DNA from up to 5 ml of blood plasma using vacuum filtration. The application of vacuum filtration technology eliminates tedious centrifugation steps and manual handling of columns during the process (e.g., disposal of collected column flowthrough). Combining NucleoSpin cfDNA Midi with the NucleoVac 96 Vacuum Manifold and the Starter Set Midi enables processing of up to 24 samples in parallel in ~90 minutes.

The purification protocol is based on the lysis of the plasma samples using Proteinase K incubation in the presence of a chaotropic salt-containing buffer. Following the initial lysis step, a binding buffer is added to the samples and the lysate is loaded onto the binding columns. After loading and vacuum filtration-driven binding of the DNA to the silica membrane, impurities are removed by several washing steps. cfDNA is eluted after a final drying step.

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Cat. #	Product	Size	Price
740303.48	NucleoSpin® cfDNA Midi	48 Preps	USD $1060.00
NucleoSpin cfDNA Midi is designed for the rapid, medium-scale preparation of highly pure cell-free DNA from up to 5 ml of blood plasma using vacuum filtration. The purification protocol involves lysis of plasma samples using Proteinase K incubation in the presence of a chaotropic salt-containing buffer. Following the initial lysis step, a binding buffer is added to the samples and the lysate is loaded onto the binding columns. After loading and vacuum filtration-driven binding of DNA to the silica membrane, impurities are removed by several washing steps and cfDNA is eluted after a final drying step. The use of vacuum filtration technology eliminates tedious centrifugation steps and manual handling of columns during processing. NucleoSpin cfDNA Midi allows for processing of up to 24 samples in parallel in 90 min. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Optimized protocol for cfDNA isolation from Cell-Free DNA BCT (Streck) tubes Optimized protocol for cfDNA isolation from Cell-Free DNA BCT (Streck) tubes. Blood was drawn from five subjects and preserved with Cell-Free DNA BCT (Streck) and EDTA tubes, respectively. cfDNA was isolated with NucleoSpin cfDNA Midi. cfDNA yields from EDTA and Streck tubes were similar due to optimization of the kit protocol for processing samples preserved in Streck tubes. DNA yields were quantified by qPCR. Back Efficient isolation of cfDNA from 2 ml of human EDTA-treated plasma Efficient isolation of cfDNA from 2 ml of human EDTA-treated plasma. cfDNA was isolated from individual EDTA plasma samples with the NucleoSpin cfDNA Midi kit and a vacuum-based kit from a competitor (Competitor Q). DNA yields were quantified by qPCR. Back NucleoSpin Plasma Midi procedure: binding and washing step NucleoSpin Plasma Midi procedure: binding and washing step Back NucleoSpin Plasma Midi procedure: drying step NucleoSpin Plasma Midi procedure: drying step Back NucleoSpin Plasma Midi procedure: elution step NucleoSpin Plasma Midi procedure: elution step Back 740303.48: NucleoSpin DNA Plasma Midi
740302.48	NucleoSpin® cfDNA Midi Core Kit	48 Preps	USD $870.00
The NucleoSpin cfDNA Midi Core Kit includes all of the components provided with the NucleoSpin cfDNA Midi kit (Cat. # 740303.48) minus the 24-Square-well Block 10 mL, and is designed for the rapid, medium-scale preparation of highly pure cell-free DNA from up to 5 ml of blood plasma using vacuum filtration. The purification protocol involves lysis of plasma samples using Proteinase K incubation in the presence of a chaotropic salt-containing buffer. Following the initial lysis step, a binding buffer is added to the samples and the lysate is loaded onto the binding columns. After loading and vacuum filtration-driven binding of DNA to the silica membrane, impurities are removed by several washing steps and cfDNA is eluted after a final drying step. The use of vacuum filtration technology eliminates tedious centrifugation steps and manual handling of columns during processing. The NucleoSpin cfDNA Midi Core Kit allows for processing of up to 24 samples in parallel in 90 min. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Optimized protocol for cfDNA isolation from Cell-Free DNA BCT (Streck) tubes Optimized protocol for cfDNA isolation from Cell-Free DNA BCT (Streck) tubes. Blood was drawn from five subjects and preserved with Cell-Free DNA BCT (Streck) and EDTA tubes, respectively. cfDNA was isolated with NucleoSpin cfDNA Midi. cfDNA yields from EDTA and Streck tubes were similar due to optimization of the kit protocol for processing samples preserved in Streck tubes. DNA yields were quantified by qPCR. Back Efficient isolation of cfDNA from 2 ml of human EDTA-treated plasma Efficient isolation of cfDNA from 2 ml of human EDTA-treated plasma. cfDNA was isolated from individual EDTA plasma samples with the NucleoSpin cfDNA Midi kit and a vacuum-based kit from a competitor (Competitor Q). DNA yields were quantified by qPCR. Back NucleoSpin Plasma Midi procedure: binding and washing step NucleoSpin Plasma Midi procedure: binding and washing step Back NucleoSpin Plasma Midi procedure: drying step NucleoSpin Plasma Midi procedure: drying step Back NucleoSpin Plasma Midi procedure: elution step NucleoSpin Plasma Midi procedure: elution step Back 740302.48: NucleoSpin DNA Plasma Midi Core Kit

Overview

Superior recovery of fragmented cell-free DNA
Parallel purification of 24 samples—special adapter set for NucleoVac 96 Vacuum Manifold
Optimized protocol for Cell-Free DNA BCT (“Streck Tubes”)

More Information

Technology	Silica membrane
Format	Midi spin columns
Starting material	1–5 ml plasma
Blood draw tubes	EDTA, Cell-Free DNA BCT (Streck)
Fragment size	≥50 bp
Typical yield	Varies depending on sample source, storage, and quality
Elution volume	200 µl (140 µl final eluate volume)
Preparation time	90 min/24 preps (EDTA plasma)

Applications

Typical downstream applications: NGS, qPCR, ddPCR
Highly efficient recovery of cell-free DNA from liquid biopsies

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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NucleoSpin cfDNA Midi

Notice to purchaser

Optimized protocol for cfDNA isolation from Cell-Free DNA BCT (Streck) tubes

Efficient isolation of cfDNA from 2 ml of human EDTA-treated plasma

NucleoSpin Plasma Midi procedure: binding and washing step

NucleoSpin Plasma Midi procedure: drying step

NucleoSpin Plasma Midi procedure: elution step

740303.48: NucleoSpin DNA Plasma Midi