Purification of viral RNA and DNA from cell-free biological fluids—NucleoSpin Virus
The NucleoSpin Virus kit is designed for efficient isolation of viral RNA and DNA from serum, plasma, or any cell-free biological fluids. RNA and DNA viruses are lysed quickly and efficiently using a lysis buffer that consists of a highly concentrated chaotropic salt solution. DNA viruses, which are usually more difficult to lyse, also require a digestion with Proteinase K, which is supplied in the kit.
The NucleoSpin Virus kit is designed for efficient isolation of viral RNA and DNA from serum, plasma, or any cell-free biological fluids. RNA and DNA viruses are lysed quickly and efficiently using a lysis buffer that consists of a highly concentrated chaotropic salt solution. DNA viruses, which are usually more difficult to lyse, also require a digestion with Proteinase K, which is supplied in the kit. Carrier RNA improves binding and recovery of dilute viral nucleic acids. A combination of the supplied lysis buffer and ethanol create appropriate conditions for nucleic acids to bind to the NucleoSpin Virus Columns. After three washing steps to remove salts, metabolites, and soluble macromolecular cellular components, the nucleic acids are eluted in water and are ready for use in downstream reactions.
Overview
- Suitable for cell-free bodily fluids, such as serum or plasma
- Efficient recovery of viral nucleic acids from a 200-µl starting volume (or up to a 400-µl starting volume with two loading steps)
- Nucleic acid isolation from human and animal samples
- Proteinase K solution included for efficient sample lysis
More Information
| Technology | Silica membrane |
| Format | Mini spin columns |
| Starting material | <200 µl serum, plasma, cell-free biological fluids (up to 400 µl with two loading steps) |
| Fragment size | 100 bp–approx. 50 kb |
| Elution volume | 30 µl |
| Preparation time | 50 min/6 preps |
| Binding capacity | 25 µg |
Applications
- Viral RNA and DNA purification from serum, plasma, and cell-free biological fluids
- Typical downstream applications include RT-PCR and enzymatic reactions
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Designed for extra small samples
Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.
Learn more All genomic DNA kitsTakara Bio USA, Inc.
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