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Nucleic acid purification

Rapid desalting and concentration of plasmid DNA preps using a vacuum manifold—NucleoSnap Finisher Midi/Maxi kits

The fastest way to desalt and concentrate DNA after your ion-exchange plasmid purification preps

Eluates from anion-exchange plasmid preparations (such as NucleoBond midipreps and maxipreps) contain high salt concentrations. Macherey-Nagel has developed a new vacuum-driven accessory, the NucleoSnap Finisher Midi/Maxi kits, that allows you to desalt and concentrate plasmid DNA in a few minutes without any loss of yield or quality. These kits are the fastest way to desalt and concentrate DNA after your NucleoBond plasmid DNA preps. NucleoSnap Finisher Midi/Maxi kits supersede the commonly used and time-consuming isopropanol precipitation and centrifugation steps by allowing rapid DNA precipitation and concentration of large volumes under vacuum.

Principle/procedure

DNA is precipitated by Precipitation Buffer FB and filtered through the specially designed matrix of the novel NucleoSnap Finisher Columns, followed by a washing step with ethanolic Wash Buffer A4 to remove salts and impurities.

To enable the use of a tabletop centrifuge, the NucleoSnap Finisher Column consists of an upper funnel component that can be separated from the lower mini spin column component by simply snapping off the funnel component. Then the mini spin column can be centrifuged in a standard tabletop centrifuge to remove residual ethanol.

Afterward, plasmid DNA can be eluted in the supplied endotoxin-free H₂O-EF. The applied elution volume can be adjusted from 100 μl to 1.5 ml according to the expected amount of plasmid DNA, in order to ensure an optimal yield and concentration.

Desalt and concentrate plasmid midipreps and maxipreps.

Figure 1. The NucleoSnap finisher midi/maxi procedure.

Application data

Ultra-fast plasmid DNA desalting and concentration.

Figure 2. Ultra-fast plasmid DNA desalting and concentration using the NucleoSnap Finisher Maxi kit. NucleoBond Xtra Maxi kit eluates were precipitated using either the NucleoSnap Finisher Maxi kit or a conventional isopropanol precipitation. Six preparations can be processed in only seven minutes with the NucleoSnap Finisher Maxi kit, about 1/10^th the time required for isopropanol precipitation.

100% DNA recovery using NucleoSnap Finisher Maxi.

Figure 3. 100% DNA recovery using the NucleoSnap Finisher Maxi kit. DNA yields in NucleoBond Xtra Maxi kit eluates were measured before and after precipitation with the NucleoSnap Finisher Maxi kit and isopropanol. With the NucleoSnap Finisher kits, 100% DNA recovery can be achieved, compared to 90% for isopropanol.

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Product

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Price

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Quantity

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740434.50

NucleoSnap® Finisher Midi

50 Preps

USD $250.00

Eluates from anion exchange plasmid preparations (such as NucleoBond midipreps and maxipreps) contain high salt concentrations. Macherey-Nagel has developed a new vacuum-driven accessory, the NucleoSnap Finisher Midi/Maxi kits, that allow you to desalt and concentrate plasmid DNA in a few minutes without any loss of yield or quality. These kits are the fastest way to desalt and concentrate DNA after your NucleoBond plasmid DNA preps. NucleoSnap Finisher Midi/Maxi kits supersede the commonly used and time-consuming isopropanol precipitation and centrifugation steps by allowing rapid DNA precipitation and concentration of large volumes under vacuum.

Cat. # 740434.50 includes sufficient quantities of reagents and materials for processing of 50 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

740435.50

NucleoSnap® Finisher Maxi

50 Preps

USD $291.00

Cat. # 740435.50 includes sufficient quantities of reagents and materials for processing of 50 samples.

Notice to purchaser

740435.10

NucleoSnap® Finisher Maxi

10 Preps

USD $63.00

Cat. # 740435.10 includes sufficient quantities of reagents and materials for processing of 10 samples.

Notice to purchaser

Required Products

Cat. #	Product	Size	Price
740414.10	NucleoBond® Xtra Maxi	10 Preps	USD $283.00
NucleoBond Xtra Maxi (10) 10 preps for the isolation of plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A Documents You May Also Like Image Data Resources Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer. Back 740414.10: NucleoBond Xtra Maxi Back Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit).
740424.10	NucleoBond® Xtra Maxi EF	10 Preps	USD $397.00
NucleoBond Xtra Maxi EF 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A Documents You May Also Like Image Data Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back 740424.10: NucleoBond Xtra Maxi EF
740414.50	NucleoBond® Xtra Maxi	50 Preps	USD $1340.00
NucleoBond Xtra Maxi 50 preps for the isolation of plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A Documents You May Also Like Image Data Resources Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer. Back 740414.50: NucleoBond Xtra Maxi Back Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit).
740414.100	NucleoBond® Xtra Maxi	100 Preps	USD $2480.00
Use NucleoBond Xtra Maxi and Maxi Plus to purify plasmid DNA in 30 min without the use of vacuum or centrifugation, Typical yield >1,000 µg of ultra-pure plasmid DNA. Documents You May Also Like Image Data Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer. Back 740414.100: NucleoBond Xtra Maxi Back Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit).
740424.50	NucleoBond® Xtra Maxi EF	50 Preps	USD $1980.00
NucleoBond Xtra Maxi EF 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A Documents You May Also Like Image Data Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer. Back 740424.50: NucleoBond Xtra Maxi EF
740574	NucleoBond® PC 500	10 Preps	USD $237.00
NucleoBond PC 500 (10) 10 preps for the isolation of plasmid DNA - NucleoBond AX 500 Columns, NucleoBond Folded Filters, buffers, RNase A Documents You May Also Like Image Data Back The NucleoBond PC plasmid purification protocol The NucleoBond PC plasmid purification protocol. Back Transfection of HeLa cells with NucleoBond-purified plasmid DNA Transfection of HeLa cells with NucleoBond-purified plasmid DNA. Visualization was performed with GFP (Green Fluorescent Protein). Data kindly provided by Dr. Niller, Mr. Vogel, Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany. Back 740574: NucleoBond PC 500
740574.25	NucleoBond® PC 500	25 Preps	USD $555.00
NucleoBond PC 500 (25) 25 preps for the isolation of plasmid DNA - NucleoBond AX 500 Columns, NucleoBond Folded Filters, buffers, RNase A Documents You May Also Like Image Data Back The NucleoBond PC plasmid purification protocol The NucleoBond PC plasmid purification protocol. Back Transfection of HeLa cells with NucleoBond-purified plasmid DNA Transfection of HeLa cells with NucleoBond-purified plasmid DNA. Visualization was performed with GFP (Green Fluorescent Protein). Data kindly provided by Dr. Niller, Mr. Vogel, Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany. Back 740574.25: NucleoBond PC 500
740574.50	NucleoBond® PC 500	50 Preps	USD $1080.00
NucleoBond PC 500 (50) 50 preps for the isolation of plasmid DNA - NucleoBond AX 500 Columns, NucleoBond Folded Filters, buffers, RNase A Documents You May Also Like Image Data Back The NucleoBond PC plasmid purification protocol The NucleoBond PC plasmid purification protocol. Back Transfection of HeLa cells with NucleoBond-purified plasmid DNA Transfection of HeLa cells with NucleoBond-purified plasmid DNA. Visualization was performed with GFP (Green Fluorescent Protein). Data kindly provided by Dr. Niller, Mr. Vogel, Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany. Back 740574.50: NucleoBond PC 500
740574.100	NucleoBond® PC 500	100 Preps	USD $2120.00
NucleoBond PC 500 (100) 100 preps for the isolation of plasmid DNA - NucleoBond AX 500 Columns, NucleoBond Folded Filters XL, buffers, RNase A Documents You May Also Like Image Data Back The NucleoBond PC plasmid purification protocol The NucleoBond PC plasmid purification protocol. Back Transfection of HeLa cells with NucleoBond-purified plasmid DNA Transfection of HeLa cells with NucleoBond-purified plasmid DNA. Visualization was performed with GFP (Green Fluorescent Protein). Data kindly provided by Dr. Niller, Mr. Vogel, Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany. Back 740574.100: NucleoBond PC 500

740434.10

NucleoSnap® Finisher Midi

10 Preps

USD $54.00

Cat. # 740434.10 includes sufficient quantities of reagents and materials for processing of 10 samples.

Notice to purchaser

Required Products

Cat. #	Product	Size	Price
740571	NucleoBond® PC 20	20 Preps	USD $159.00
NucleoBond PC 20 (20) 20 preps for the isolation of plasmid DNA - NucleoBond AX 20 Columns, buffers, RNase A Documents You May Also Like Image Data Back 740571: NucleoBond PC 20
740571.100	NucleoBond® PC 20	100 Preps	USD $630.00
NucleoBond PC 20 (100) 100 preps for the isolation of plasmid DNA - NucleoBond AX 20 Columns, buffers, RNase A Documents You May Also Like Image Data Back Transfection of HeLa cells with NucleoBond-purified plasmid DNA Transfection of HeLa cells with NucleoBond-purified plasmid DNA. Visualization was performed with GFP (Green Fluorescent Protein). Data kindly provided by Dr. Niller, Mr. Vogel, Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany. Back 740571.100: NucleoBond PC 20
740410.10	NucleoBond® Xtra Midi	10 Preps	USD $124.00
NucleoBond Xtra Midi (10) 10 preps for the isolation of plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Documents You May Also Like Image Data Back Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits. Plasmid DNA was isolated following each manufacturer's protocol using the maximum culture volume with high plasmid content. The yield of plasmid DNA was determined after DNA precipitation. NucleoBond Xtra Midi/Maxi (midiprep and maxiprep kits) provide up to 60% time savings and up to 100% higher yields compared to competitors’ kits. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume. Back Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit).
740410.50	NucleoBond® Xtra Midi	50 Preps	USD $585.00
NucleoBond Xtra Midi (50) 50 preps for the isolation of plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Documents You May Also Like Image Data Resources Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back 740410.50: NucleoBond Xtra Midi Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume. Back Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit).
740410.100	NucleoBond® Xtra Midi	100 Preps	USD $1075.00
Use Midi prep and Midi prep Xtra Plus to purify plasmid DNA in 30 min without the use of vacuum or centrifugation. Typical yield >500 µg of ultra-pure plasmid DNA. Documents Image Data Back Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits. Plasmid DNA was isolated following each manufacturer's protocol using the maximum culture volume with high plasmid content. The yield of plasmid DNA was determined after DNA precipitation. NucleoBond Xtra Midi/Maxi (midiprep and maxiprep kits) provide up to 60% time savings and up to 100% higher yields compared to competitors’ kits. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume. Back Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi Higher yields than Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi. Plasmid DNA was purified from bacterial cultures using NucleoBond Xtra Midi, NucleoBond Xtra Maxi, and corresponding midiprep and maxiprep kits from Qiagen following the protocols for each kit (in each case, the maximum input volume specified by the corresponding kit protocol was processed). Yields of plasmid DNA were 60–100% higher with the NucleoBond kits than the Qiagen kits (Q Midi = Qiagen Plasmid Midi Kit, Q Maxi = Qiagen Plasmid Maxi Kit). Back 740410.10: NucleoBond Xtra Midi
740420.10	NucleoBond® Xtra Midi EF	10 Preps	USD $186.00
NucleoBond Xtra Midi EF (10) 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Documents You May Also Like Image Data Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor. Back 740420.10: NucleoBond Xtra Midi EF Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume.
740420.50	NucleoBond® Xtra Midi EF	50 Preps	USD $850.00
NucleoBond Xtra Midi EF (50) 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A Documents You May Also Like Image Data Back The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution. Back The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin) The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer. Back NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE. Back The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor. Back 740420.50: NucleoBond Xtra Midi EF Back Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume.
740573	NucleoBond® PC 100	20 Preps	USD $224.00
NucleoBond PC 100 (20) 20 preps for the isolation of plasmid DNA - NucleoBond AX 100 Columns, Folded Filters, buffers, RNase A Documents You May Also Like Image Data Back The NucleoBond PC plasmid purification protocol The NucleoBond PC plasmid purification protocol. Back Transfection of HeLa cells with NucleoBond-purified plasmid DNA Transfection of HeLa cells with NucleoBond-purified plasmid DNA. Visualization was performed with GFP (Green Fluorescent Protein). Data kindly provided by Dr. Niller, Mr. Vogel, Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany. Back 740573: NucleoBond PC 100
740573.100	NucleoBond® PC 100	100 Preps	USD $1020.00
NucleoBond PC 100 (100) 100 preps for the isolation of plasmid DNA - NucleoBond AX 100 Columns, NucleoBond Folded Filters, buffers, RNase A Documents You May Also Like Image Data Back The NucleoBond PC plasmid purification protocol The NucleoBond PC plasmid purification protocol. Back Transfection of HeLa cells with NucleoBond-purified plasmid DNA Transfection of HeLa cells with NucleoBond-purified plasmid DNA. Visualization was performed with GFP (Green Fluorescent Protein). Data kindly provided by Dr. Niller, Mr. Vogel, Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany. Back 740573.100: NucleoBond PC 100

Overview

No time-consuming isopropanol precipitation
New column design (snap-off column) for vacuum processing of large sample volumes
Process 12 samples in less than 10 minutes
100% plasmid DNA recovery

More Information

Technology	Silica-membrane technology
Processing	Vacuum processing (e.g., using NucleoVac 24 Vacuum Manifold), or elution by centrifugation
Format	Snap-off column
Compatibility	Eluates from NucleoBond preparations: Xtra Midi (EF), Xtra Maxi (EF), PC 20, PC 100, PC 500 (EF)
Starting material	DNA eluates from e.g., anion-exchange purification kits
Vector size	<25 kb
Typical recovery	90–100%
Elution volume	≥100 µl
Preparation time	<10 min/12 preps
Binding capacity	1.5 mg

Applications

Quick concentration and desalination of plasmid and cosmid DNA eluates obtained by anion-exchange chromatography
Typical downstream applications: transfection, in vitro transcription, automated or manual sequencing, cloning, hybridization, and PCR

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Isolate up to 700 μg of transfection-grade plasmid DNA in only 35 minutes

Isolate up to 700 µg of transfection-grade plasmid DNA in only 35 minutes

Using novel chemistry and a unique snap-off column design, NucleoSnap Plasmid Midi provides rapid purification of up to 700 µg of transfection-grade plasmid. The snap-off columns allow processing of lysate from large 50-ml culture volumes, and multiple samples can be rapidly processed by vacuum using our NucleoVac 24 Vacuum Manifold.

Learn more Other plasmid kits

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Rapid desalting and concentration of plasmid DNA preps using a vacuum manifold—NucleoSnap Finisher Midi/Maxi kits

Principle/procedure

Application data

Notice to purchaser

Ultra-fast plasmid DNA desalting and concentration using the NucleoSnap Finisher Maxi kit

100% DNA recovery using the NucleoSnap Finisher Maxi kit

The NucleoSnap Finisher Midi/Maxi procedure

740434.50: NucleoSnap Finisher Midi

Notice to purchaser

Ultra-fast plasmid DNA desalting and concentration using the NucleoSnap Finisher Maxi kit

100% DNA recovery using the NucleoSnap Finisher Maxi kit

The NucleoSnap Finisher Midi/Maxi procedure

740435.50: NucleoSnap Finisher Maxi

Notice to purchaser

740435.10: NucleoSnap Finisher Maxi

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

740414.10: NucleoBond Xtra Maxi

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

740424.10: NucleoBond Xtra Maxi EF

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

740414.50: NucleoBond Xtra Maxi

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

740414.100: NucleoBond Xtra Maxi

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

740424.50: NucleoBond Xtra Maxi EF

The NucleoBond PC plasmid purification protocol

Transfection of HeLa cells with NucleoBond-purified plasmid DNA

740574: NucleoBond PC 500

The NucleoBond PC plasmid purification protocol

Transfection of HeLa cells with NucleoBond-purified plasmid DNA

740574.25: NucleoBond PC 500

The NucleoBond PC plasmid purification protocol

Transfection of HeLa cells with NucleoBond-purified plasmid DNA

740574.50: NucleoBond PC 500

The NucleoBond PC plasmid purification protocol

Transfection of HeLa cells with NucleoBond-purified plasmid DNA

740574.100: NucleoBond PC 500

Notice to purchaser

740434.10: NucleoSnap Finisher Midi

740571: NucleoBond PC 20

Transfection of HeLa cells with NucleoBond-purified plasmid DNA

740571.100: NucleoBond PC 20

Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

740410.50: NucleoBond Xtra Midi

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

Higher plasmid yields in less time—in comparison to competitors’ anion exchange-based kits

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

Higher yields versus Qiagen with NucleoBond Xtra Midi and NucleoBond Xtra Maxi

740410.10: NucleoBond Xtra Midi

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor

740420.10: NucleoBond Xtra Midi EF

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor

740420.50: NucleoBond Xtra Midi EF