NucleoSpin Inhibitor Removal
A common challenge associated with homebrew DNA extraction protocols and phenol-based methods is that they often fail to efficiently remove inhibitory compounds such as humic acids, polyphenols, heme, and tannins that interfere with downstream PCR-based applications. The NucleoSpin Inhibitor Removal kit provides a simple and effective solution for such cases, recovering high yields of ready-to-amplify DNA from diverse inputs in only 15 minutes.
A common challenge associated with homebrew DNA extraction protocols and phenol-based methods is that they often fail to efficiently remove inhibitory compounds such as humic acids, polyphenols, heme, and tannins that interfere with downstream PCR-based applications. The NucleoSpin Inhibitor Removal kit provides a simple and effective solution for such cases, recovering high yields of ready-to-amplify DNA from diverse inputs in only 15 minutes.
NucleoSpin Inhibitor Removal employs silica membrane technology in a familiar mini spin column format to enable rapid recovery of high-quality DNA from inputs up to 100 µl. Alternate protocols are provided for optimal processing of samples with varying levels of humic substances and other inhibitory factors. Purified DNA can be eluted in volumes ranging from 50–100 µl, with typical recoveries >75%.
Overview
- Efficient removal of PCR inhibitors from contaminated samples
- Superior DNA recovery vs. competitor kits
- Alternate protocols provided for optimal processing of different sample types
More Information
| Technology | Silica membrane |
| Starting material | Contaminated DNA extracts from diverse samples |
| Sample amount | Up to 100 µl |
| Fragment size | 200 bp–approximately 50 kbp |
| DNA recovery |
Typically >75% |
| Elution volume | 50–100 µl |
| Processing time | 15 min/6 preps |
Applications
- Removal of PCR inhibitors from DNA eluates
- Typical downstream applications: PCR, qPCR, NGS
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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