Sequencing reaction purification—NucleoSEQ columns
Unincorporated dye terminators negatively affect the analysis of sequencing results. An excess of dye terminators causes so-called "dye blobs", which result in a partly unreadable sequence.
NucleoSEQ columns remove unincorporated dye terminators, so the subsequent analysis is of high quality with long read length and minimized background. NucleoSEQ columns are designed for fast, effective, and cost-efficient sequencing reaction cleanup. The spin columns are prefilled with a dry size-exclusion matrix for efficient removal of dye terminators (e.g., BigDye terminators).
Unincorporated dye terminators negatively affect the analysis of sequencing results. An excess of dye terminators causes so-called "dye blobs", which result in a partly unreadable sequence.
NucleoSEQ columns remove unincorporated dye terminators, so the subsequent analysis is of high quality with long read length and minimized background. NucleoSEQ columns are designed for fast, effective, and cost-efficient sequencing reaction cleanup. The spin columns are prefilled with a dry size-exclusion matrix for efficient removal of dye terminators (e.g., BigDye terminators). The gel-filtration material consists of spheres with uniform pores and separates molecules according to molecular weight. After applying the sequencing reaction to the NucleoSEQ column, the small dye terminators and other impurities (e.g., salts, nucleotides, primers, and traces of organic solvents) are retained in the pores while labeled DNA fragments are excluded and recovered in the flowthrough with high yield.
In order to achieve long-term storage at room temperature, NucleoSEQ columns are prefilled with dry gel-filtration resin. The matrix can easily be hydrated by adding water followed by an incubation period (>30 minutes). Hydrated columns are ready to use and can be stored at 4°C for 14 days. A short centrifugation step removes the remaining storage buffer. After loading the sample onto the column, followed by a second centrifugation step, the DNA fragments of interest are recovered in the flowthrough.
Overview
In accordance with Macherey-Nagel´s Quality Management System, each lot of product is tested against predetermined specifications to ensure consistent product quality.
More Information
Applications
- Dye-terminator removal
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Designed for extra small samples
Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.
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