High-throughput isolation of genomic DNA from forensic samples—NucleoSpin 8/96 Trace
NucleoSpin 8/96 Trace kits are designed for the preparation of highly pure genomic DNA from small amounts of any tissue, cell, or forensic sample (e.g., buccal swabs, blood spots, cigarette filters). The obtained DNA can be used directly as template for PCR.
NucleoSpin 8/96 Trace kits are designed for the preparation of highly pure genomic DNA from small amounts of any tissue, cell, or forensic sample (e.g., buccal swabs, blood spots, cigarette filters). The obtained DNA can be used directly as template for PCR.
Lysis is achieved by incubation of samples in a solution containing chaotropic ions in the presence of Proteinase K. If subsequent separation of the lysate from the sample material is difficult, the lysate can be separated from the forensic material under vacuum or centrifugation using the NucleoSpin Trace Filter Plate (not included in the kits). Appropriate conditions for binding of DNA to the silica membrane in the NucleoSpin Trace Binding Strip/Binding Plate are created by addition of isopropanol to the lysate. The binding process is reversible and specific to nucleic acids. Contaminants are removed by two washing steps with ethanolic buffer. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer.
Typically, 1–2 µg genomic DNA can be purified from buccal swabs. The final concentration of eluted DNA ranges from 10–20 ng/µl (depending on the elution buffer volume) and the A260/280 is 1.8–1.9. The kits can be used manually under centrifugation or vacuum and can also be automated on common liquid handling instruments.
Overview
- Time-saving parallel isolation of genomic DNA from forensic samples
- Processing is possible under vacuum or by centrifugation
- Suitable for manual and automated processing
- Innovative MN Wash Plate minimizes risk of cross-contamination
More Information
| NucleoSpin 8 Trace | NucleoSpin 96 Trace | |
| Technology | Silica membrane | Silica membrane |
| Format | 8-well strips | 96-well plates |
| Processing | Manual or automated, vacuum or centrifugation |
Manual or automated, vacuum or centrifugation |
| Starting material | Forensic samples (e.g., buccal swabs, blood spots, cigarette filters) |
Forensic samples (e.g., buccal swabs, blood spots) |
| Typical yield | 1–2 µg | 1–2 µg |
| A260/280 | 1.8–1.9 | 1.8–1.9 |
| Elution volume | 50–100 µl | 50–100 µl |
| Preparation time | 30 min/6 strips | 70 min/plate |
| Binding capacity | 20 µg | 20 µg |
Applications
Manual or automated isolation of genomic DNA from forensic samples:
- Buccal swabs
- Dried blood spots
- Cigarette filters
Purified DNA is ready to use for any kind of enzymatic reactions (e.g., STR analysis).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Designed for extra small samples
Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.
Learn more All genomic DNA kitsTakara Bio USA, Inc.
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