Genomic DNA isolation from blood—NucleoSpin Blood kits
NucleoSpin 8/96 Blood QuickPure kits are designed for rapid parallel isolation of genomic DNA from whole blood, serum, plasma, and bodily fluids (e.g., amniotic fluid, liquor, etc.)—in flexible 8-well strip or high-throughput 96-well plate formats which have been optimized for manual use. Animal or human blood samples and fresh or frozen material are processed conveniently via centrifugation of NucleoSpin Blood QuickPure Binding Strips/Plates.
NucleoSpin 8/96 Blood QuickPure kits are designed for rapid parallel isolation of genomic DNA from whole blood, serum, plasma, and bodily fluids (e.g. amniotic fluid, liquor, etc.)—in flexible 8-well strip or high-throughput 96-well plate formats which have been optimized for manual use. Animal or human blood samples and fresh or frozen material are processed conveniently via centrifugation of NucleoSpin Blood QuickPure Binding Strips/Plates.
The NucleoSpin Blood QuickPure method features a specially treated silica membrane which speeds up the procedure by allowing a combined washing/drying step. This reduces the number of washing and drying steps from three to only one, minimizing hands-on time. Lysis is achieved by incubation of whole blood in a solution containing large amounts of chaotropic ions in the presence of Proteinase K. Appropriate conditions for DNA binding to the silica membrane of the NucleoSpin Blood QuickPure Binding Strips/Plates are created by addition of ethanol to the lysate. Pure genomic DNA is eluted under low ionic strength conditions in a slightly alkaline elution buffer, and can be used directly for PCR, Southern blotting, or any kind of enzymatic reaction.
The NucleoSpin 8/96 Blood kits are designed for the rapid parallel isolation of genomic DNA from whole blood or cultured cells. After lysis of blood cells, genomic DNA is selectively bound to a silica membrane, while contaminants are thoroughly washed away. Elution is possible even under vacuum without cross-contamination. Due to its plate design and the capability for performing the complete procedure under vacuum, NucleoSpin 8/96 Blood can be easily adapted to common liquid-handling instruments for fully automated processing without manual interactions.
The NucleoSpin 96 Blood Core kit provides the buffers, Proteinase K, and NucleoSpin Blood Binding Plates only. Accessory plates (e.g., lysis plates, elution plates) are not provided with the core kit. The user can select additional consumables from a variety of suitable accessory plates according to individual needs, for the highest flexibility.
Overview
- NucleoSpin 8/96 Blood QuickPure
- Time-saving isolation of genomic DNA from whole blood
- Optimized for manual use in a centrifuge
- Reduced number of washing and drying steps minimizes hands-on time
- Consistently high DNA yields
- NucleoSpin 8/96 Blood
- Optimized for manual or automated use under vacuum
- Improved flow rate reduces blockage of wells (reliable results, no cross-contamination)
- Innovative MN Wash Plate minimizes risk of cross-contamination
- Complete processing possible at room temperature
- Consistently high yields
More Information
|
NucleoSpin 8 Blood QuickPure |
NucleoSpin 96 Blood QuickPure |
|
| Technology |
Silica membrane |
|
| Format |
8-well strips |
96-well plates |
| Processing |
Manual, centrifugation |
|
| Starting material |
Human or animal whole blood, treated with EDTA, citrate, heparin, CPDA |
|
| Fragment size |
300 bp–>30 kb |
|
| Typical yield |
4–6 µg |
|
| A260/280 |
1.6–1.9 |
|
| Elution volume |
75–100 µl |
|
| Preparation time |
60 min/12 strips |
60 min/2 plates |
| Binding capacity |
60 µg |
|
Product overview: NucleoSpin 8/96 Blood
|
NucleoSpin 8 Blood |
NucleoSpin 96 Blood |
|
| Technology |
Silica membrane |
|
| Format |
8-well strips |
96-well plates |
| Processing |
Manual or automated, optimized for vacuum processing |
|
| Starting material |
Whole blood treated with EDTA, citrate, heparin, CPDA, human or animal blood |
|
| Fragment size |
300 bp–>30 kb |
|
| Typical yield |
4–6 µg |
|
| A260/280 |
1.8–1.9 |
|
| Elution volume |
100 µl |
|
| Preparation time |
35 min/6 strips |
70 min/plate |
| Binding capacity |
20 µg |
|
Applications
- NucleoSpin 8/96 Blood QuickPure can be used for manual isolation of genomic DNA from:
- Whole blood
- Serum
- Plasma
- Buffy coats
- Platelets
- Body fluids
- Cultured cells
- NucleoSpin 8/96 Blood QuickPure can be used for automated isolation of genomic DNA from:
- Whole blood
- Cultured cells
- Obtained DNA is ready to use for:
- PCR
- Southern blotting
- Enzymatic reactions
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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