mPlum fluorescent protein

Far-red proteins such as mPlum avoid the natural green autofluorescence found in plants and animals and are preferred for in vivo imaging. Living Colors mPlum is a fruit fluorescent protein which was developed in Dr. Roger Tsien’s lab (Shaner et al. 2004; Wang et al. 2004; Shu et al. 2006) by directed mutagenesis of a monomeric mutant of DsRed (Campbell et al. 2002). It has demonstrated stable expression and performed successfully in numerous fusion applications.

Cat. #

Product

Size

Price

License

Quantity

Details

632527

pmPlum Vector

20 ug

USD $529.00

License Statement

ID Number
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pmPlum Vector product License Agreement can be found by clicking here.
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
44	mPlum and mRaspberry Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,393,923 and 8,932,859.

pmPlum encodes mPlum, a mutant derived from the tetrameric Discosoma sp. red fluorescent protein, DsRed. In this vector, the mPlum coding sequence is flanked by MCS regions, making it easy to excise the gene for use in other cloning applications. pmPlum is primarily intended to serve as a source of mPlum cDNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

Overview

Far-red fluorescent protein for in vivo imaging
mPlum excitation and emission maxima: 590 and 649 nm, respectively
See which antibodies can be use to detect mPlum on Western blot or ICC

More Information

Applications

In vivo imaging
Fusions
Protein localization studies

References

Campbell, R. E. et al. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. U. S. A. 99, 7877-82 (2002).

Shaner, N. C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22, 1567–72 (2004).

Shu, X., Shaner, N. C., Yarbrough, C. A., Tsien, R. Y. & Remington, S. J. Novel chromophores and buried charges control color in mFruits. Biochemistry 45, 9639–9647 (2006).

Wang, L., Jackson, W. C., Steinbach, P. a & Tsien, R. Y. Evolution of new nonantibody proteins via iterative somatic hypermutation. Proc. Natl. Acad. Sci. U. S. A. 101, 16745–16749 (2004).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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