RNA isolation reagent for miRNA and total RNA—NucleoZOL
NucleoZOL is a highly efficient RNA-isolation reagent for extracting both small and large RNAs from a variety of sample types, such as cultured cells, animal and plant tissue, yeast, bacteria, and viruses. A simple, one-phase extraction with NucleoZOL eliminates the need for an inconvenient two-phase separation using chloroform, which, in turn, minimizes the risk of sample loss and phenol carryover. DNA, protein, and other contaminants are removed by precipitation.
NucleoZOL is a highly efficient RNA-isolation reagent for extracting both small and large RNAs from a variety of sample types, such as cultured cells, animal and plant tissue, yeast, bacteria, and viruses. A simple, one-phase extraction with NucleoZOL eliminates the need for an inconvenient two-phase separation using chloroform, which, in turn, minimizes the risk of sample loss and phenol carryover. DNA, protein, and other contaminants are removed by precipitation. NucleoZOL can be used to isolate total RNA in a single fraction, or miRNA and large RNA in two separate fractions. The NucleoZOL procedure can also be used in combination with NucleoSpin RNA columns.
The NucleoSpin RNA Set for NucleoZOL is a mini spin kit designed to combine NucleoZOL RNA extraction with NucleoSpin RNA purification. The kit contains silica membrane-based columns and the required buffers. After a sample is lysed in NucleoZOL (not provided in the mini spin kit), and contaminating DNA and proteins are pelleted, the supernatant is mixed with an optimized binding buffer for efficient binding of miRNA and large RNA to the silica membrane. A convenient bind-wash-elute procedure allows highly efficient isolation of pure RNA (miRNA and large RNA).
Overview
- Simple procedure with no need for a phase separation using chloroform
- High yields of highly pure RNA from a wide variety of sample types
- Isolation of miRNA and large RNA in a single fraction or two separate fractions
- One-phase RNA extraction combined with mini spin column purification
More Information
NucleoZOL
| Technology | One-phase extraction |
| Format | Reagent |
| Starting material | <1 x 107 cultured cells, bacteria, and yeast <100 mg human/animal/plant tissue <0.4 ml (viral) fluids |
| Fragment size | miRNA (10–200 nt), large RNA (>200 nt) |
| Typical yield (total RNA) | Liver: 6–8 µg/mg tissue Kidney, spleen: 3–4 µg/mg tissue Muscle, brain, lung: 0.5–1.5 µg/mg Cultured cells: 4–10 µg/106 cells |
| Typical yield (large RNA) | Liver: 5–7 µg/mg tissue Kidney, spleen: 3–4 µg/mg tissue Muscle, brain, lung: 0.5–1.5 µg/mg Cultured cells: 3–8 µg/106 cells |
| A260/A280 | 1.8–2.1 |
| Typical RIN (RNA Integrity Number) | >9 |
| Elution volume | Flexible |
| Preparation time | <1 hr |
NucleoSpin RNA Set for NucleoZOL
| Technology | Silica membrane technology |
| Format | Mini spin columns |
| Starting material | ≤500 µl NucleoZOL* sample |
| Fragment size | miRNA (10–200 nt), large RNA (>200 nt) |
| Typical recovery | 85–95% |
| Typical RIN (RNA Integrity Number) | Depending on sample quality (no significant loss of RIN detected) |
| Elution volume | 60 µl |
| Preparation time | <1 hr |
| Binding capacity | 200 µg |
*NucleoZOL not included.
Applications
- RNA isolation from cultured cells, animal and plant tissue, yeast, bacteria, and viruses
- Real-time RT-PCR, Northern blotting, primer extension, array technology, and RNase protection assays
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Universal reagent for RNA isolation
NucleoZOL is a highly efficient RNA isolation reagent for extracting high-quality miRNA and total RNA from a variety of starting materials, including cells, tissues, and liquids of human or animal origin, as well as plants, yeast, bacteria, viruses, and other sources.
See the data RNA purification kitsTakara Bio USA, Inc.
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