Universal kit for challenging plant and fungal samples—NucleoSpin RNA Plant and Fungi
Plant and fungal material is diverse in terms of structure, cell wall composition, biochemical compounds, and types of organs. Common products for isolating RNA from plant material usually work well with samples such as Arabidopsis leaves, but fail to efficiently purify RNA from challenging samples. The NucleoSpin RNA Plant and Fungi kit was developed to efficiently purify high-integrity RNA from samples rich in secondary metabolites, sugar, starch, or other compounds that interfere with common nucleic acid purification methods. The kit enables RNA isolation from diverse plant organs such as seeds, roots, stems, needles, and others. It can also be used for standard samples such as Arabidopsis leaves.
Overview
- Tailored protocols for diverse starting materials
- Convenient handling and efficient isolation of high-integrity RNA
- Includes NucleoSpin RNA Plant and Fungi Filters for lysate clearing
More Information
Technology | Silica membrane |
Format | Mini spin columns |
Starting material | <500 mg plant/fungal material |
Fragment size | >200 nt |
Typical yield | 20–70 µg |
A260/A280 | 1.9–2.1 |
A260/A230 | ~2 |
Typical RIN (RNA integrity number) | 7–9 |
Elution volume | 50 µl |
Preparation time | 25 min/6 preps |
Binding capacity | 200 µg |
Applications
- RNA isolation from plant and fungi
- Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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