One-step RT-PCR—gene-specific RT—Titanium One-Step RT-PCR Kit
The Titanium One-Step RT-PCR Kit provides an easy-to-use, streamlined procedure for performing reverse transcription-PCR. The entire reaction is performed in a single tube, eliminating cross-contamination problems. Along with convenience, this kit offers unparalleled sensitivity and efficiency and is ideal for comparing gene expression levels across different samples. With its high sensitivity, you can rely on the Titanium One-Step RT-PCR Kit for all analytical RT-PCR applications.
The Titanium One-Step RT-PCR Kit provides an easy-to-use, streamlined procedure for performing reverse transcription-PCR. The entire reaction is performed in a single tube, eliminating cross-contamination problems. Along with convenience, this kit offers unparalleled sensitivity and efficiency and is ideal for comparing gene expression levels across different samples. With its high sensitivity, you can rely on the Titanium One-Step RT-PCR Kit for all analytical RT-PCR applications.
Improved one-step method
Traditional methods use separate steps for reverse transcription and PCR, thus requiring more hands-on time and allowing for potential sample crossover and contamination. In contrast, the Titanium One-Step RT-PCR Kit allows you to go straight from RT incubation to PCR cycling without opening tubes or adding reagents. This kit includes reagents that ensure superior performance, allowing you to:
- Minimize problems associated with RNA secondary structure. The thermostabilizing reagent allows reverse transcription to be performed at 50°C, providing increased specificity when used with gene-specific primers.
- Perform hot-start PCR for enhanced specificity and sensitivity. The Titanium Taq RT Enzyme Mix includes TaqStart Antibody for built-in hot-start PCR (Gerard et al. 1993; Kellogg et al. 1994).
One-step RT-PCR is the best method for working with multiple RNA samples. However, for amplifying multiple fragments from a single RNA source, two-step RT-PCR can actually be more efficient because only a single reverse transcription reaction mix is required. For two-step applications, we recommend our Advantage RT-for-PCR Kit.
Overview
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Allows qualitative analysis of gene expression from multiple samples
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Produces results from as little as 10 pg of total RNA
- Provided in a convenient single-tube format
- Easy-to-use one-step format minimizes the risk of cross contamination
More Information
Applications
- One-step RT-PCR
- Endpoint RT-PCR
- Gene expression analysis
- First-strand cDNA synthesis
References
Gerard, G. F., et al., Reverse transcriptase (EC 2.7.7.49): the use of cloned maloney murine leukemia virus reverse transcriptase to synthesize DNA from RNA. In Burrell,M.M. (ed.). Methods in Molecular Biology: Enzymes of Molecular Biology. (Humana Press, Inc., Totowa, NJ), 16:73–93 (1993).
Kellogg, D. E., et al., TaqStart Antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. BioTechniques 16:1134–1137 (1994).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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