Ultrapure SMART MMLV Reverse Transcriptase for RT-PCR

SMART MMLV Reverse Transcriptase is an ultra-pure Moloney Murine Leukemia Virus reverse transcriptase designed to virtually eliminate contaminating RNases and other nucleases that are often introduced into the RT reaction with the enzyme. SMART MMLV RT helps prevent degradation of template RNA, while allowing the synthesis of high quality, full-length cDNA (up to 11.7 kb) from almost any transcript.

Cat. #	Product	Size	Price
639524	SMART® MMLV Reverse Transcriptase	20,000 Units	USD $260.00
SMART MMLV Reverse Transcriptase is an ultra-pure, recombinant Moloney Murine Leukemia Virus reverse transcriptase (MMLV RT). This highly purified protein is a fully active RT that is free of exogenous RNases and other nucleases. As a result, SMART MMLV RT is able to synthesize a higher percentage of full-length cDNAs, making it the ideal enzyme for a wide range of applications. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back SMART MMLV RT has less RNase activity than the competition SMART MMLV RT has less RNase activity than the competition. An RNA ladder was incubated with 200 units of either SMART MMLV RT (Lanes 1 & 2), or another supplier’s MMLV RT (Lanes 3 & 4) and AMV RT (Lanes 5 & 6) at 37°C for 1 hr, and then analyzed by formaldehyde/agarose gel electrophoresis. Lane 7: negative control (buffer only, no enzyme). Lane 8: positive control. . Back SMART MMLV RT is ideal for SMART mRNA amplification SMART MMLV RT is ideal for SMART mRNA amplification. Full-length sense mRNA was amplified from 0.1 μg and 1 μg of Human Placenta Total RNA using our SMART mRNA Amplification Kit (which uses T7 RNA Polymerase). The amplified mRNA was then analyzed on a denaturing agarose gel. Lane 1: RNA ladder; Lanes 2 & 3: mRNA amplified from 1 μg of total RNA; Lanes 4 & 5: mRNA amplified from 0.1 μg of total RNA; Lane 6: Negative Control (–RNA). Back SMART MMLV RT synthesizes long, full-length cDNAs SMART MMLV RT synthesizes long, full-length cDNAs. SMART MMLV RT was used to reverse transcribe first-strand cDNA from Human Brain Total RNA using oligo(dT)18 primers (Panel A). The resulting single-stranded cDNA was then serially diluted fivefold (corresponding to 25 ng–1.6 pg of RNA) and analyzed by qPCR using QTaq DNA Polymerase Mix and gene-specific primers that amplified a 226-bp region near the 5' end of NF1 (Panel B). The successful amplification of this region indicates that SMART MMLV RT was able to reverse transcribe at least 11.7 kb of the NF1 gene. Back Comparison of SMARTScribe RT and SMART MMLV RT Comparison of SMARTScribe RT and SMART MMLV RT. Back 639524: SMART MMLV Reverse Transcriptase
639523	SMART® MMLV Reverse Transcriptase	8,000 Units	USD $116.00
SMART MMLV Reverse Transcriptase is an ultra-pure, recombinant Moloney Murine Leukemia Virus reverse transcriptase (MMLV RT). This highly purified protein is a fully active RT that is free of exogenous RNases and other nucleases. As a result, SMART MMLV RT is able to synthesize a higher percentage of full-length cDNAs, making it the ideal enzyme for a wide range of applications. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back SMART MMLV RT has less RNase activity than the competition SMART MMLV RT has less RNase activity than the competition. An RNA ladder was incubated with 200 units of either SMART MMLV RT (Lanes 1 & 2), or another supplier’s MMLV RT (Lanes 3 & 4) and AMV RT (Lanes 5 & 6) at 37°C for 1 hr, and then analyzed by formaldehyde/agarose gel electrophoresis. Lane 7: negative control (buffer only, no enzyme). Lane 8: positive control. . Back SMART MMLV RT is ideal for SMART mRNA amplification SMART MMLV RT is ideal for SMART mRNA amplification. Full-length sense mRNA was amplified from 0.1 μg and 1 μg of Human Placenta Total RNA using our SMART mRNA Amplification Kit (which uses T7 RNA Polymerase). The amplified mRNA was then analyzed on a denaturing agarose gel. Lane 1: RNA ladder; Lanes 2 & 3: mRNA amplified from 1 μg of total RNA; Lanes 4 & 5: mRNA amplified from 0.1 μg of total RNA; Lane 6: Negative Control (–RNA). Back SMART MMLV RT synthesizes long, full-length cDNAs SMART MMLV RT synthesizes long, full-length cDNAs. SMART MMLV RT was used to reverse transcribe first-strand cDNA from Human Brain Total RNA using oligo(dT)18 primers (Panel A). The resulting single-stranded cDNA was then serially diluted fivefold (corresponding to 25 ng–1.6 pg of RNA) and analyzed by qPCR using QTaq DNA Polymerase Mix and gene-specific primers that amplified a 226-bp region near the 5' end of NF1 (Panel B). The successful amplification of this region indicates that SMART MMLV RT was able to reverse transcribe at least 11.7 kb of the NF1 gene. Back Comparison of SMARTScribe RT and SMART MMLV RT Comparison of SMARTScribe RT and SMART MMLV RT. Back 639523: SMART MMLV Reverse Transcriptase
639522	SMART® MMLV Reverse Transcriptase	2,000 Units	USD $75.00
SMART MMLV Reverse Transcriptase is an ultra-pure, recombinant Moloney Murine Leukemia Virus reverse transcriptase (MMLV RT). This highly purified protein is a fully active RT that is free of exogenous RNases and other nucleases. As a result, SMART MMLV RT is able to synthesize a higher percentage of full-length cDNAs, making it the ideal enzyme for a wide range of applications. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Comparison of SMARTScribe RT and SMART MMLV RT Comparison of SMARTScribe RT and SMART MMLV RT. Back 639522: SMART MMLV Reverse Transcriptase

Overview

Synthesize long, full-length cDNA up to 11.7 kb
Amplify rare transcripts
Maintain the complexity of the original RNA
Robust ultrapure MMLV with consistent performance

More Information

Applications

Microarray
Real-time PCR/qPCR
Gene expression analysis

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Ultrapure SMART MMLV Reverse Transcriptase for RT-PCR

Notice to purchaser

SMART MMLV RT has less RNase activity than the competition

SMART MMLV RT is ideal for SMART mRNA amplification

SMART MMLV RT synthesizes long, full-length cDNAs

Comparison of SMARTScribe RT and SMART MMLV RT

639524: SMART MMLV Reverse Transcriptase

Notice to purchaser

SMART MMLV RT has less RNase activity than the competition

SMART MMLV RT is ideal for SMART mRNA amplification

SMART MMLV RT synthesizes long, full-length cDNAs

Comparison of SMARTScribe RT and SMART MMLV RT

639523: SMART MMLV Reverse Transcriptase

Notice to purchaser

Comparison of SMARTScribe RT and SMART MMLV RT

639522: SMART MMLV Reverse Transcriptase

Overview

More Information

Applications

Additional product information