Oligo modification: Uni-Link AminoModifier
Uni-Link AminoModifier is a cyanoethyl phosphoramidite that directly incorporates primary aliphatic amines into oligonucleotides, providing an efficient way to directly label oligos with various reporter molecules (Nelson, Kent, and Muthini 1992; Nelson and Muthini 1993; Nelson et al. 1993; Nelson and Muthini 1993). Uni-Link AminoModifier possesses the patented 2-aminobutyl-1,3-propanediol (ABPD) backbone, which offers the following distinct labeling advantages:
Uni-Link AminoModifier is a cyanoethyl phosphoramidite that directly incorporates primary aliphatic amines into oligonucleotides, providing an efficient way to directly label oligos with various reporter molecules (Nelson, Kent, and Muthini 1992; Nelson and Muthini 1993; Nelson et al. 1993; Nelson and Muthini 1993). Uni-Link AminoModifier possesses the patented 2-aminobutyl-1,3-propanediol (ABPD) backbone, which offers the following distinct labeling advantages:
- Multiple labels can be incorporated because phosphoramidites do not truncate chain elongation at 5' termini.
- Natural 3-carbon internucleotide phosphodiester distance is maintained, providing better hybridization and annealing properties than those obtained with other labeling methods.
- Attached label is separated from the phosphodiester connection point to reduce steric hindrance. The ABPD backbone has a 7-atom spacer arm that separates the label from the connection point.
- Label incorporation can be easily measured by standard trityl cation assays because phosphoramidites possess a dimethoxytrityl (DMT)-protected hydroxyl group.
Please inquire about bulk orders or custom synthesis requests at Technical_Support@takarabio.com.
Overview
- Incorporate single or multiple labels during oligonucleotide synthesis
- Place labels at the 5' end, internally, or at the 3' end
- Maintain natural 3-carbon internucleotide phosphate distance
- Obtain very high coupling efficiencies on any commercially available synthesizer
More Information
Applications
- Oligo labeling (reagents)
- Nucleic acid modifications
- Nucleic acid chemistry (reagents)
- Nucleic acid labeling (reagents)
- Nucleic acid synthesis (reagents)
References
Nelson, P.S., Kent, M., & Muthini, S. Oligonucleotide labeling methods 3. Direct labeling of oligonucleotides employing a novel, non-nucleosidic, 2-aminobutyl-1,3-propanediol backbone. Nucleic Acids Res. 20, 6253-6259 (1992).
Nelson, P. & Muthini, S. OligoTechniques 1, 1-4 (1993).
Nelson, P., et al. OligoTechniques 1, 5-7 (1993).
Nelson, P. & Muthini, S. Biomedical Products 18, 40 (1993).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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