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Nucleic acid purification and fractionation: CHROMA SPIN columns

CHROMA SPIN columns are packed with gel filtration resin and enable rapid purification and size selection of nucleic acid samples. The columns can be used to purify single- or double-stranded DNA or RNA from contaminants such as salts, solvents, enzymes, or proteins. Molecules larger than the particular matrix pore size are eluted out of the column while smaller molecules are retained inside the column matrix. As a result, these columns are ideal for size fractionation of libraries or for removal of primers.

Available in three different sterile buffers

TE buffer: ideal for routine DNA applications
STE (0.1 M NaCl + TE) buffer: used for applications requiring a higher salt concentration to prevent ionic interaction between molecules
DEPC-treated water (+ 0.1 mM EDTA): useful for RNA purification and applications requiring a completely nuclease-free environment

Choose from six different column pore sizes

CHROMA SPIN-10, -30, -100, -200, -400, and -1000. All columns, matrices, and buffers have been autoclaved to destroy nucleases. For optimal recovery and purification, there should be at least a threefold difference between the size of the desired nucleic acid molecules and the size of the contaminants.

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Cat. #	Product	Size	Price
636096	CHROMA SPIN™-200+DEPC-H2O Columns	20 Each	USD $365.00
CHROMA SPIN-200+DEPC-H2O Columns are used for the purification of nucleic acids larger than 200 bp in size. Matrix is in DEPC-treated, autoclaved H₂O + 0.1 mM EDTA (pH 8.0). Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Schematic of the CHROMA SPIN purification protocol Schematic of the CHROMA SPIN purification protocol. Back Size selection for removal of small oligonucleotides Size selection for removal of small oligonucleotides. A 53-mer, 33-mer, and 6-mer were independently labeled with [γ³²P]ATP using T4 kinase. The mixture of labeled oligonucleotides was applied to a CHROMA SPIN-30 Column and spun briefly. A portion of the purified mixture was electrophoresed on a 20% acrylamide/ 7 M urea gel. The gel was exposed to x-ray film at room temperature for 30 min. Lane 1: mixture before column purification. Lane 2: mixture after column purification Back CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples. They are ideal for size fractionation of libraries or for removal of primers. Back 636096: CHROMA SPIN-200+DEPC-H2O Columns
636093	CHROMA SPIN™-1000+DEPC-H2O Columns	20 Each	USD $365.00
CHROMA SPIN-1,000+DEPC-H2O Columns are used for the purification of nucleic acids larger than 1,000 bp in size. Matrix is in DEPC-treated, autoclaved H₂O + 0.1 mM EDTA (pH 8.0). Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Schematic of the CHROMA SPIN purification protocol Schematic of the CHROMA SPIN purification protocol. Back Size selection for removal of small oligonucleotides Size selection for removal of small oligonucleotides. A 53-mer, 33-mer, and 6-mer were independently labeled with [γ³²P]ATP using T4 kinase. The mixture of labeled oligonucleotides was applied to a CHROMA SPIN-30 Column and spun briefly. A portion of the purified mixture was electrophoresed on a 20% acrylamide/ 7 M urea gel. The gel was exposed to x-ray film at room temperature for 30 min. Lane 1: mixture before column purification. Lane 2: mixture after column purification Back CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples. They are ideal for size fractionation of libraries or for removal of primers. Back 636093: CHROMA SPIN-1000+DEPC-H2O Columns
636090	CHROMA SPIN™-100+DEPC-H2O Columns	50 Each	USD $673.00
CHROMA SPIN-100+DEPC-H2O Columns are used for the purification of nucleic acids larger than 100 bp in size. Matrix is in DEPC-treated, autoclaved H₂O + 0.1 mM EDTA (pH 8.0). Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Schematic of the CHROMA SPIN purification protocol Schematic of the CHROMA SPIN purification protocol. Back Size selection for removal of small oligonucleotides Size selection for removal of small oligonucleotides. A 53-mer, 33-mer, and 6-mer were independently labeled with [γ³²P]ATP using T4 kinase. The mixture of labeled oligonucleotides was applied to a CHROMA SPIN-30 Column and spun briefly. A portion of the purified mixture was electrophoresed on a 20% acrylamide/ 7 M urea gel. The gel was exposed to x-ray film at room temperature for 30 min. Lane 1: mixture before column purification. Lane 2: mixture after column purification Back CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples. They are ideal for size fractionation of libraries or for removal of primers. Back 636090: CHROMA SPIN-100+DEPC-H2O Columns
636087	CHROMA SPIN™-30+DEPC-H2O Columns	50 Each	USD $673.00
CHROMA SPIN-30+DEPC-H2O Columns are used for the purification of nucleic acids larger than 30 bp in size. Matrix is in DEPC-treated, autoclaved H₂O + 0.1 mM EDTA (pH 8.0). Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Schematic of the CHROMA SPIN purification protocol Schematic of the CHROMA SPIN purification protocol. Back Size selection for removal of small oligonucleotides Size selection for removal of small oligonucleotides. A 53-mer, 33-mer, and 6-mer were independently labeled with [γ³²P]ATP using T4 kinase. The mixture of labeled oligonucleotides was applied to a CHROMA SPIN-30 Column and spun briefly. A portion of the purified mixture was electrophoresed on a 20% acrylamide/ 7 M urea gel. The gel was exposed to x-ray film at room temperature for 30 min. Lane 1: mixture before column purification. Lane 2: mixture after column purification Back CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples. They are ideal for size fractionation of libraries or for removal of primers. Back 636087: CHROMA SPIN-30+DEPC-H2O Columns
636082	CHROMA SPIN™+TE-200 Columns	50 Each	USD $673.00
CHROMA SPIN+TE-200 Columns are used for the purification of nucleic acids larger than 200 bp in size. Matrix is in TE buffer (10 mM Tris-HCl [pH 8.0], and 1 mM EDTA). Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Schematic of the CHROMA SPIN purification protocol Schematic of the CHROMA SPIN purification protocol. Back Size selection for removal of small oligonucleotides Size selection for removal of small oligonucleotides. A 53-mer, 33-mer, and 6-mer were independently labeled with [γ³²P]ATP using T4 kinase. The mixture of labeled oligonucleotides was applied to a CHROMA SPIN-30 Column and spun briefly. A portion of the purified mixture was electrophoresed on a 20% acrylamide/ 7 M urea gel. The gel was exposed to x-ray film at room temperature for 30 min. Lane 1: mixture before column purification. Lane 2: mixture after column purification Back CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples. They are ideal for size fractionation of libraries or for removal of primers. Back 636082: CHROMA SPIN+TE-200 Columns
636079	CHROMA SPIN™+TE-1000 Columns	50 Each	USD $673.00
CHROMA SPIN+TE-1000 Columns are used for the purification of nucleic acids larger than 1,000 bp in size. Matrix is in TE buffer (10 mM Tris-HCl [pH 8.0], and 1 mM EDTA). Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Schematic of the CHROMA SPIN purification protocol Schematic of the CHROMA SPIN purification protocol. Back Size selection for removal of small oligonucleotides Size selection for removal of small oligonucleotides. A 53-mer, 33-mer, and 6-mer were independently labeled with [γ³²P]ATP using T4 kinase. The mixture of labeled oligonucleotides was applied to a CHROMA SPIN-30 Column and spun briefly. A portion of the purified mixture was electrophoresed on a 20% acrylamide/ 7 M urea gel. The gel was exposed to x-ray film at room temperature for 30 min. Lane 1: mixture before column purification. Lane 2: mixture after column purification Back CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples. They are ideal for size fractionation of libraries or for removal of primers. Back 636079: CHROMA SPIN+TE-1000 Columns
636076	CHROMA SPIN™+TE-400 Columns	50 Each	USD $673.00
CHROMA SPIN+TE-400 Columns are used for the purification of nucleic acids larger than 400 bp in size. Matrix is in TE buffer (10 mM Tris-HCl [pH 8.0], and 1 mM EDTA). Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Schematic of the CHROMA SPIN purification protocol Schematic of the CHROMA SPIN purification protocol. Back Size selection for removal of small oligonucleotides Size selection for removal of small oligonucleotides. A 53-mer, 33-mer, and 6-mer were independently labeled with [γ³²P]ATP using T4 kinase. The mixture of labeled oligonucleotides was applied to a CHROMA SPIN-30 Column and spun briefly. A portion of the purified mixture was electrophoresed on a 20% acrylamide/ 7 M urea gel. The gel was exposed to x-ray film at room temperature for 30 min. Lane 1: mixture before column purification. Lane 2: mixture after column purification Back CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples. They are ideal for size fractionation of libraries or for removal of primers. Back 636076: CHROMA SPIN+TE-400 Columns
636073	CHROMA SPIN™+TE-100 Columns	50 Each	USD $673.00
CHROMA SPIN+TE-100 Columns are used for the purification of nucleic acids larger than 100 bp in size. Matrix is in TE buffer (10 mM Tris-HCl [pH 8.0], and 1 mM EDTA). Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Schematic of the CHROMA SPIN purification protocol Schematic of the CHROMA SPIN purification protocol. Back Size selection for removal of small oligonucleotides Size selection for removal of small oligonucleotides. A 53-mer, 33-mer, and 6-mer were independently labeled with [γ³²P]ATP using T4 kinase. The mixture of labeled oligonucleotides was applied to a CHROMA SPIN-30 Column and spun briefly. A portion of the purified mixture was electrophoresed on a 20% acrylamide/ 7 M urea gel. The gel was exposed to x-ray film at room temperature for 30 min. Lane 1: mixture before column purification. Lane 2: mixture after column purification Back CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples. They are ideal for size fractionation of libraries or for removal of primers. Back 636073: CHROMA SPIN+TE-100 Columns
636072	CHROMA SPIN™+TE-100 Columns	20 Each	USD $365.00
CHROMA SPIN+TE-100 Columns are used for the purification of nucleic acids larger than 100 bp in size. Matrix is in TE buffer (10 mM Tris-HCl [pH 8.0], and 1 mM EDTA). Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Schematic of the CHROMA SPIN purification protocol Schematic of the CHROMA SPIN purification protocol. Back Size selection for removal of small oligonucleotides Size selection for removal of small oligonucleotides. A 53-mer, 33-mer, and 6-mer were independently labeled with [γ³²P]ATP using T4 kinase. The mixture of labeled oligonucleotides was applied to a CHROMA SPIN-30 Column and spun briefly. A portion of the purified mixture was electrophoresed on a 20% acrylamide/ 7 M urea gel. The gel was exposed to x-ray film at room temperature for 30 min. Lane 1: mixture before column purification. Lane 2: mixture after column purification Back CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples. They are ideal for size fractionation of libraries or for removal of primers. Back 636072: CHROMA SPIN+TE-100 Columns
636069	CHROMA SPIN™+TE-30 Columns	50 Each	USD $673.00
CHROMA SPIN+TE-30 Columns are used for the purification of nucleic acids larger than 30 bp in size. Matrix is in TE buffer (10 mM Tris-HCl [pH 8.0], and 1 mM EDTA). Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Schematic of the CHROMA SPIN purification protocol Schematic of the CHROMA SPIN purification protocol. Back Size selection for removal of small oligonucleotides Size selection for removal of small oligonucleotides. A 53-mer, 33-mer, and 6-mer were independently labeled with [γ³²P]ATP using T4 kinase. The mixture of labeled oligonucleotides was applied to a CHROMA SPIN-30 Column and spun briefly. A portion of the purified mixture was electrophoresed on a 20% acrylamide/ 7 M urea gel. The gel was exposed to x-ray film at room temperature for 30 min. Lane 1: mixture before column purification. Lane 2: mixture after column purification Back CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples. They are ideal for size fractionation of libraries or for removal of primers. Back 636069: CHROMA SPIN+TE-30 Columns

Overview

Adaptable—one system for any application
Efficient—purify samples in 10 minutes
Simple—apply sample and spin

More Information

Applications

Nucleic acid purification
Removal of contaminants
Buffer exchange
Library construction
Microarray spotting
PCR
Cloning

Principal CHROMA SPIN column applications

CHROMA SPIN Series	Purify oligos/primers after labeling reactions (bases)	DNA/RNA lengths (bases)	Remove primers/linkers digestion fragments (bases)	Remove proteins/enzymes (kDa)
CHROMA SPIN-10	>15	—	—	—
CHROMA SPIN-30	—	>35	<9	<30
CHROMA SPIN-100	—	>140	<30	<250
CHROMA SPIN-200	—	>300	<50	<1,000
CHROMA SPIN-400	—	>600	<100	<8,000
CHROMA SPIN-1000	—	>1,350	<300	all sizes

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Nucleic acid purification and fractionation: CHROMA SPIN columns

Available in three different sterile buffers

Choose from six different column pore sizes

Notice to purchaser

Schematic of the CHROMA SPIN purification protocol

Size selection for removal of small oligonucleotides

CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples

636096: CHROMA SPIN-200+DEPC-H2O Columns

Notice to purchaser

Schematic of the CHROMA SPIN purification protocol

Size selection for removal of small oligonucleotides

CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples

636093: CHROMA SPIN-1000+DEPC-H2O Columns

Notice to purchaser

Schematic of the CHROMA SPIN purification protocol

Size selection for removal of small oligonucleotides

CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples

636090: CHROMA SPIN-100+DEPC-H2O Columns

Notice to purchaser

Schematic of the CHROMA SPIN purification protocol

Size selection for removal of small oligonucleotides

CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples

636087: CHROMA SPIN-30+DEPC-H2O Columns

Notice to purchaser

Schematic of the CHROMA SPIN purification protocol

Size selection for removal of small oligonucleotides

CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples

636082: CHROMA SPIN+TE-200 Columns

Notice to purchaser

Schematic of the CHROMA SPIN purification protocol

Size selection for removal of small oligonucleotides

CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples

636079: CHROMA SPIN+TE-1000 Columns

Notice to purchaser

Schematic of the CHROMA SPIN purification protocol

Size selection for removal of small oligonucleotides

CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples

636076: CHROMA SPIN+TE-400 Columns

Notice to purchaser

Schematic of the CHROMA SPIN purification protocol

Size selection for removal of small oligonucleotides

CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples

636073: CHROMA SPIN+TE-100 Columns

Notice to purchaser

Schematic of the CHROMA SPIN purification protocol

Size selection for removal of small oligonucleotides

CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples

636072: CHROMA SPIN+TE-100 Columns

Notice to purchaser

Schematic of the CHROMA SPIN purification protocol

Size selection for removal of small oligonucleotides

CHROMA SPIN Columns are packed with gel filtration resin to rapidly purify and size select nucleic acid samples

636069: CHROMA SPIN+TE-30 Columns

Overview

More Information

Applications

Principal CHROMA SPIN column applications

Additional product information