SeqAmp DNA Polymerase

SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot-start capability that is well-suited for use with many SMARTer kits for next-generation sequencing. This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions.

Cat. #	Product	Size	Price
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $408.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $147.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back 638504: SeqAmp DNA Polymerase
638526	SeqAmp™ CB PCR Buffer	50 Rxns	USD $35.00
SeqAmp™ CB PCR Buffer is designed for improved compatibility with DNA purification using Agencourt AMPure beads after amplification with SeqAmp DNA Polymerase and works with SMARTer® next-generation sequencing kits. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back SeqAmp CB PCR buffer improves bead-pellet formation Improved bead-pellet formation with new SeqAmp CB PCR buffer. The PCR buffer included in the Pico v2 kit was re-formulated to allow for faster, tighter bead-pellet formation. Following magnetic separation for a fixed period, bead pellets formed in the new SeqAmp CB buffer (right) are tighter than those formed in the original PCR buffer (left). Tighter bead pellets tend to dry more evenly and are easier to resuspend than pellets that are broader and more diffuse. Back 638526: SeqAmp CB PCR Buffer

Overview

Recommended for use with SMARTer next-generation sequencing kits.

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Applications

High-fidelity PCR enzyme sold as part of several SMARTer kits for next-generation sequencing, used to amplify purified first-strand cDNA into RNA-seq libraries.
The SeqAmp CB PCR Buffer (Cat. # 638526) is an optional buffer that may substitute for the SeqAmp PCR Buffer for easier bead resuspension when using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing. Note that for the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian, the SeqAmp CB PCR Buffer is included as part of the kit.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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SeqAmp DNA Polymerase

Notice to purchaser

638509: SeqAmp DNA Polymerase

Notice to purchaser

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

638504: SeqAmp DNA Polymerase

Notice to purchaser

SeqAmp CB PCR buffer improves bead-pellet formation

638526: SeqAmp CB PCR Buffer

Overview

More Information

Applications

Additional product information