External Standard Kit (Lambda PolyA) for qPCR
With real-time PCR (qPCR), target-gene expression levels are often normalized to the expression of a reference gene to obtain relative quantification of expression. This normalization strategy corrects for differences in the amount of input RNA and variation in reaction efficiency. A housekeeping gene that is stably expressed in all the samples being tested is commonly used as the reference. However, in some experimental systems, it may be difficult to identify a gene with stable expression. In such cases, an external standard RNA can be added to the reaction to serve as a reference.
With real-time PCR (qPCR), target-gene expression levels are often normalized to the expression of a reference gene to obtain relative quantification of expression. This normalization strategy corrects for differences in the amount of input RNA and variation in reaction efficiency. A housekeeping gene that is stably expressed in all the samples being tested is commonly used as the reference. However, in some experimental systems, it may be difficult to identify a gene with stable expression. In such cases, an external standard RNA can be added to the reaction to serve as a reference.
External Standard Kit (Lambda PolyA) for qPCR provides an external reference for qPCR normalization. The kit contains the reference RNA (λ polyA+ RNA-A) and primers for real-time PCR detection. A fixed amount of the λ polyA RNA can be directly added to RNA samples before cDNA synthesis with oligo-dT. Subsequently, intercalating green dye-based qPCR detection can be used to quantify the amount of λ polyA RNA in each sample.
More Information
Applications
- qPCR normalization
Components
| Real Time Primers for λ polyA (10 µM) | 200 µl each |
| EASY Dilution (for Real Time PCR)* | 1 ml |
| λ polyA+ RNA-A (10 ng/µl) | 15 µl |
* EASY Dilution (for Real Time PCR) is also available separately (Cat. # 9160)
Storage
- Real Time Primer for λ polyA and EASY Dilution (for Real Time PCR): −20°C
- λ polyA+ RNA-A: −80°C
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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