Cryonase Cold-Active Nuclease

Cryonase Cold-Active Nuclease is a recombinant endonuclease originally derived from a psychrophile, Shewanella sp., and purified from E. coli. This endonuclease enzyme can complete digestion of all types of DNA and RNA (single-stranded, double-stranded, linear or circularized) at cold temperatures and even in samples placed on ice. As a result, Cryonase Cold-Active Nuclease is frequently used during DNA digestion of mixed samples, such as those that include heat-labile proteins.

Cat. #	Product	Size	Price
2670B	Cryonase™ Cold-Active Nuclease	50,000 Units	USD $513.00
Cryonase Cold-Active Nuclease is a recombinant endonuclease originating from a psychrophile, Shewanella sp., which is expressed in and purified from E. coli. This enzyme can digest all types of DNA and RNA (single-stranded, double-stranded, linear or circularized), even on ice. Cryonase is useful for digestion of nucleic acids in samples including heat-labile substances such as proteins. Cat. # 2670B contains 5 of Cat. # 2670A. Please refer to Cat. # 2670A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 2670B: Cryonase Cold-Active Nuclease
2670A	Cryonase™ Cold-Active Nuclease	10,000 Units	USD $114.00
Cryonase Cold-Active Nuclease is a recombinant endonuclease originating from a psychrophile, Shewanella sp., which is expressed in and purified from E. coli. This enzyme can digest all types of DNA and RNA (single-stranded, double-stranded, linear or circularized), even on ice. Cryonase is useful for digestion of nucleic acids in samples including heat-labile substances such as proteins. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 2670A: Cryonase Cold-Active Nuclease

More Information

Applications

Digestion of all types of DNA
Digestion of all types of RNA
Enzymatic and other heat-labile reactions

Purity

Protease activity is less than the detection limit
E. coli genomic DNA contamination is less than the detection limit

Buffer

Supplied in a buffer (20 mM NaCl, 10 mM Tris-HCl (pH 7.5), 50% glycerol, 2mM MgCl₂)

Product citations

Baldermann, S. et al. Functional characterization of a carotenoid cleavage dioxygenase 1 and its relation to the carotenoid accumulation and volatile emission during the floral development of Osmanthus fragrans Lour. J. Exp. Bot. 61(11):2967–2977 (2010).

Yang, H. et al. Full Genome Sequence of Bluetongue Virus Serotype 4 from China. J. Virol. 86(23):13122–13123 (2012).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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