AAV purification kits
AAV vectors have emerged as highly effective gene delivery tools for therapeutic research, in part due to the ability to target specific cell/tissue types with a variety of recombinant AAV serotypes (Büning 2019). Generating AAV vectors with good efficacy requires a method that yields vectors of high purity. However, many currently available schemas are serotype dependent, requiring separate method development/optimization for each vector. Click "More" to see how our kits provide a solution.
AAV vectors have emerged as highly effective gene delivery tools for therapeutic research, in part due to the ability to target specific cell/tissue types with a variety of recombinant AAV serotypes (Büning 2019). Generating AAV vectors with good efficacy requires a method that yields vectors of high purity. However, many currently available schemas are serotype dependent, requiring separate method development/optimization for each vector.
Our "All Serotypes" kits solve this issue through the use of a simple precipitation and filtration protocol that can purify AAV particles with any capsid (i.e., from any serotype).
AAVpro Cell & Sup. Purification Kit Maxi (All Serotypes) allows the researcher to purify virus particles from both AAV-producing cells and the culture supernatant, providing higher virus yields. Each kit/pack contains sufficient reagents for four virus preps, each from a culture size of five T225 flasks. See table below for additional culture volume information. This kit contains:
- AAVpro Cell & Sup. Extraction/Concentration Pack Maxi (All Serotypes) allows researchers to extract virus particles from both AAV-producing cells and the culture supernatant, providing higher virus yields. Each pack contains sufficient reagents to prepare four preps of AAV particle extracts, each from a culture size of five T225 flasks. AAV viral particles can be further purified using the AAVpro Purification Pack Maxi (All Serotypes) (Cat. # 6678).
- AAVpro Purification Pack Maxi (All Serotypes) allows researchers to purify four virus preps, each from a culture size of five T225 flasks of AAV-producing cells and culture supernatant.
The AAVpro Purification Kit Maxi (All Serotypes) allows the researcher to purify four virus preps, each from a culture size of five T225 flasks. See table below for additional culture volume information.
Alternatively, the AAVpro Purification Kit Midi (All Serotypes) version of the kit provides four preps, each from a culture size of one T225 flask (or four 10-cm dishes). See table below for additional culture volume information.
The AAVpro Purification Kit (AAV2) is serotype dependent, and thus restricted to AAV2 since the protocol uses affinity-based purification. With this kit, you can purify two AAV2 preps, each from a culture size of five T225 flasks. See table below for additional culture volume information.
Overview
- Simple workflow: optimized protocol that eliminates the need for freeze-thaw cycles and ultracentrifugation steps
- High purity for reliable data: purified AAV is suitable for in vivo research
- Serotype agnostic: precipitation and filtration protocol that can purify AAV particles from any serotype (Cat. # 6666, 6675, and 6676 only)
Note: The AAVpro Purification (All Serotype) Kits (Cat. # 6666, 6675, and 6676) include Cryonase Cold-Active Nuclease (Cat. # 2670A and 2670B). This nuclease is not included with the AAVpro Purification Kit (AAV2; Cat. # 6232) but can be purchased separately to remove residual nucleic acid as an optional step.
| Product | Cat. # | Serotype(s) | Size | # of flasks or dishes per purification |
|---|---|---|---|---|
| AAVpro Cell & Sup. Purification Kit Maxi (All Serotypes) | 6676 | All | 4 purifications |
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| AAVpro Purification Kit Maxi (All Serotypes) | 6666 | All | 4 purifications |
|
| AAVpro Purification Kit Midi (All Serotypes) | 6675 | All | 4 purifications |
|
| AAVpro Purification Kit (AAV2) | 6232 | AAV2 | 2 purifications |
|
More Information
References
Büning, H. and Srivastava, A. Capsid Modifications for Targeting and Improving the Efficacy of AAV Vectors. Mol. Ther. Methods Clin. Dev. 12, 248–265 (2019).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Fast and simple AAV purification
Need to purify AAV particles quickly or without ultracentrifugation? Watch these videos to learn about our AAVpro "All Serotypes" kit, a simple column-based purification method for easy purification of AAV particles from virus-producing cells.
- Unique AAV vector extraction method eliminates the need for ultracentrifugation
- Kits include all reagents needed to purify AAV particles from vector-producing cells
- Protocol yields highly pure AAV particles that can be used directly for in vivo transduction, or transduction of cultured cells
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