Adenoviral transduction
Adenoviral gene transfer is one of the most reliable methods for introducing genes into mammalian cells. Because infection by adenovirus is not cell-cycle dependent, you can deliver your gene to primary as well as transformed cell lines. Compared to other viral systems, adenoviruses are ideal tools for achieving high expression in mammalian cells because of the ability to infect with a high MOI following infection; therefore, your target gene is expressed at high levels.
Adenoviral gene transfer is one of the most reliable methods for introducing genes into mammalian cells. Because infection by adenovirus is not cell-cycle dependent, you can deliver your gene to primary as well as transformed cell lines. Compared to other viral systems, adenoviruses are ideal tools for achieving high expression in mammalian cells because of the ability to infect with a high MOI following infection; therefore, your target gene is expressed at high levels. The adenovirus genome does not integrate into the genome of the target cells, so expression is transient in rapidly dividing cells, however, in slowly dividing and non-dividing cells expression can be long-lived. Additionally, adenoviruses are capable of infecting a wide variety of proliferating and quiescent cell types from many different animal species, including humans, nonhuman primates, pigs, rodents, mice, and rabbits.
Our adenoviral transduction line is a suite of products for highly efficient adenoviral expression and packaging, purification, and titration.
Featured product
Adenoviral titration by quantitative PCR
View data comparing titrating adenovirus using qPCR to other methods.
Adenovirus product pages
Fastest & easiest adenoviral vector system ever
Until now, the main drawback of commercially supplied adenoviral vector systems has been the need to use complex cloning procedures to overcome the difficulties with cloning into large (~34 kb) plasmids. Procedures have included precloning into shuttle vectors and subcloning through multiple steps and multiple different strains of E. coli, all of which increase hands-on time and leave more room for error. At Takara Bio, our Adeno-X virologists thought "wouldn't it be great if you could clone directly into the adenoviral plasmid just like any plasmid?" They then harnessed the power of In-Fusion HD Cloning technology to make this happen.
How it works Tet-inducible versionAdenoviral learning center
Recombinant adenovirus learning center
Tools and tech notes related to recombinant adenovirus transduction.
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