Osteocalcin ELISA kit: Gla-type osteocalcin (Gla-OC) EIA Kit
The Takara Bio osteocalcin ELISA kit offers sensitive detection of the osteogenic marker osteocalcin (OC). OC, also known as gamma-carboxyglutamic acid protein, is a small (5.9 kDa), vitamin K-dependent, hydroxyapatite (Ca2+)-binding protein synthesized exclusively by osteoblasts and odontoblasts. The tissue-specific expression of OC makes it an excellent osteogenic marker for measuring osteoblast activity during bone formation.
The Takara Bio osteocalcin ELISA kit offers sensitive detection of the osteogenic marker osteocalcin (OC). OC, also known as gamma-carboxyglutamic acid protein, is a small (5.9 kDa), vitamin K-dependent, hydroxyapatite (Ca2+)-binding protein synthesized exclusively by osteoblasts and odontoblasts. The tissue-specific expression of OC makes it an excellent osteogenic marker for measuring osteoblast activity during bone formation. Osteocalcin is also a hormone that controls the regulation of glucose and fat deposition and plays a role in male fertility. Three gamma-carboxyglutamic acid (Gla) residues at positions 17, 21, and 24 of the protein are responsible for binding calcium. OC calcium binding is also required for such biological activities as activation of the blood coagulation cascade. Carboxylated OC (Gla-OC) is most likely the active form of the protein, while decarboxylated OC (Glu-OC)—which has weak hydroxyapatite affinity—represents the inactive form. Gla-OC versus Glu-OC serum levels correlate well with bone formation; thus, the Takara Bio human osteocalcin ELISA kit provides a better analysis of bone growth and remodeling than conventional assays that do not differentiate between the active and inactive protein forms.
The Gla-Type Osteocalcin (Gla-OC) EIA Kit is a 96-well in vitro enzyme immunoassay kit for the quantitative determination of human Gla-OC in serum, cultured cell extract, cell culture supernatant, and other biological fluids. It is a solid phase sandwich EIA utilizing two mouse monoclonal Gla-OC antibodies: one of which is coated onto the plate, and the other of which is peroxidase-labeled. This assay format permits highly sensitive detection of Gla-OC using a two-step incubation method. In the first step, biological samples are incubated in the antibody-coated microtiter plate; in the second step, the plate is washed and incubated with the peroxidase-labeled Gla-OC antibody. A substrate is added, and the reaction between the peroxidase and substrate (H2O2, TMBZ) results in color development. The amount of sample Gla-OC is determined by absorbance measurement using an EIA plate reader. Accurate Gla-OC sample concentration can be determined by comparing its specific absorbance with that of the supplied standard on a standard curve.
Overview
- Human osteocalcin ELISA
- Highly sensitive quantitative measurement of human Gla-OC
More Information
Applications
- Quantitative measurement of human, bovine, rabbit, sheep, canine, or goat carboxylated osteocalcin
Performance characteristics
- Assay sensitivity: 0.5 ng/ml
- Sample volume per well: 100 µl
Product Citations
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Kaptoge, S. et al. Sex hormone status may modulate rate of expansion of proximal femur diameter in older women alongside other skeletal regulators. J. Clin. Endocrinol. Metab. 92, 304–13 (2007). Nakamura, K. et al. Nutrition, mild hyperparathyroidism, and bone mineral density in young Japanese women. Am. J. Clin. Nutr. 82, 1127–33 (2005).
Rubio-Gozalbo, M. E. et al. Bone mineral density in patients with classic galactosaemia. Arch. Dis. Child. 87, 57–60 (2002).
Schurgers, L. J. et al. Novel effects of diets enriched with corn oil or with an olive oil/sunflower oil mixture on vitamin K metabolism and vitamin K-dependent proteins in young men. J. Lipid Res. 43, 878–84 (2002).
Schurgers, L. J., Shearer, M. J., Hamulyák, K., Stöcklin, E. & Vermeer, C. Effect of vitamin K intake on the stability of oral anticoagulant treatment: dose-response relationships in healthy subjects. Blood 104, 2682–9 (2004).
Shimizu, N. et al. Shift of serum osteocalcin components between cord blood and blood at day 5 of life. Pediatr. Res. 52, 656–9 (2002).
Tsukamoto, Y., Ichise, H., Kakuda, H. & Yamaguchi, M. Intake of fermented soybean (natto) increases circulating vitamin K2 (menaquinone-7) and gamma-carboxylated osteocalcin concentration in normal individuals. J. Bone Miner. Metab. 18, 216–22 (2000).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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