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Chloramphenicol-resistant pUC plasmids

The chloramphenicol-resistant pUC plasmids pHSG396 and pHSG398 contain the chloramphenicol resistance gene, the pMB1-derived origin of replication (ori), and the beta-galactosidase coding gene lacZ. These plasmids also contain a pUC18-derived multiple cloning site (MCS) within the lacZ gene, enabling recombinant clones to be verified through culture plates containing IPTG and X-Gal. High target-gene expression is enabled by the presence of the lac promoter on both the pHSG396 and pHSG398 plasmids.

Both pHSG 396 and pHSG 398 can be analyzed via dideoxy DNA sequencing using an M13 primer, and large recombinant chloramphenicol resistant pUC plasmids may be analyzed using the Deletion Kit for Kilo-Sequencing.

Cat. #	Product	Size	Price
3398	pHSG 398 DNA	25 ug	USD $82.00
The chloramphenicol resistant pUC plasmid pHSG 398 contains the chloramphenicol resistance gene, the pMB1-derived origin of replication (ori), and the beta-galactosidase coding gene lacZ. It also contains a pUC18-derived multiple cloning site (MCS) within the lacZ gene, enabling recombinant clones to be verified through culture plates containing IPTG and X-Gal. High target gene expression is enabled by the presence of the lac promoter. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back 3398: pHSG 398 DNA
3396	pHSG 396 DNA	25 ug	USD $82.00
The chloramphenicol resistant pUC plasmid pHSG 396 contains the chloramphenicol resistance gene, the pMB1-derived origin of replication (ori), and the beta-galactosidase coding gene lacZ. It also contains a pUC18-derived multiple cloning site (MCS) within the lacZ gene, enabling recombinant clones to be verified through culture plates containing IPTG and X-Gal. High target gene expression is enabled by the presence of the lac promoter. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Chloramphenicol-resistant pUC vectors pHSG396 and pHSG398 Schematic diagram and MCS sequence of the chloramphenicol resistant pUC vectors pHSG396 and pHSG398. Both vectors contain the chloramphenicol resistance gene, the pMB1-derived origin of replication (ori), and the beta-galactosidase coding gene lacZ. These chloramphenicol-resistant pUC vectors also contain a pUC18-derived multiple cloning site (MCS) within the lacZ gene, enabling recombinant clones to be verified through culture plates containing IPTG and X-Gal. The MCS in the two vectors is reversed. Back 3396: pHSG 396 DNA

Overview

Form: 10 mM Tris-HCl (pH 8.0), 1 mM EDTA
Concentration: 250–1,000 µg/ml

More Information

Applications

lac promoter-enabled target gene cloning and expression
DNA sequencing using an M13 primer
Long DNA sequencing using Takara's Deletion Kit for Kilo-Sequencing

Source

pUC18 plasmid

Storage

–20°C

GenBank

Plasmid	Entry name	Accession no.
pUC18	SYNPUC18CV	L09136
pHSG399	SYNHSG399	M19087*

*Except for the MCS, pHSG396 and pHSG398 have the same sequence as pHSG399.

Chain length

pHSG396: 2,238 bp
pHSG398: 2,227 bp

References

Takeshita, S., Sato, M., Toba, M. & Masahashi, W. High-copy-number and low-copy-number plasmids for lacZα-complementation and chloramphenicol-or kanamycin-resistance selection. Gene 61, 63–74 (1987).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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