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Kanamycin resistant pUC vectors

The kanamycin resistant pUC vectors pHSG298 and pHSG299 contain the kanamycin resistance gene, the pMB1-derived origin of replication (ori), and the beta-galactosidase coding gene lacZ. These kanamycin resistant pUC vectors also contain a pUC19-derived multiple cloning site (MCS) within the lacZ gene, enabling recombinant clones to be verified through culture plates containing IPTG and X-Gal. High target gene expression is enabled by the presence of the lac promoter in both the pHSG298 and pHSG299 vectors ...

Both pHSG298 and pHSG299 can be analyzed via dideoxy DNA sequencing using an M13 primer, and large recombinant chloramphenicol resistant pUC vectors may be analyzed using the Deletion Kit for Kilo-Sequencing (Cat. # 6030).

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Cat. #	Product	Size	Price
3299	pHSG 299 DNA	25 ug	USD $76.00
pHSG299 is a kanamycin-resistant pUC cloning vector which contains the same consensus multicloning site as pUC19 (though Hind III and Sma I sites are not available). Using this vector, it is possible to transfer a DNA fragment from pUC18 to pUC19 without the preparation of DNA fragments. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back 3299: pHSG 299 DNA
3298	pHSG 298 DNA	25 ug	USD $76.00
pHSG298 is a kanamycin-resistant pUC cloning vector which contains the same consensus multicloning site as pUC18 (though Hind III and Sma I sites are not available). Using this vector, it is possible to transfer a DNA fragment from pUC19 to pUC18 without the preparation of DNA fragments. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back pHSG Vector Map pHSG Vector Map. Back 3298: pHSG 298 DNA

Overview

Form: 10 mM Tris-HCl (pH 8.0), 1 mM EDTA

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Applications

lac promoter-enabled target gene cloning and expression
DNA sequencing using an M13 primer
Long DNA sequencing using the Deletion Kit for Kilo-Sequencing

Storage

–20°C

Notes

The pHSG299 DNA (Cat.# 3299) sequence differs from the sequence of pHSG299 registered with GenBank at the following positions:

1663 C → deletion
1808 C → T
2228–2229 AT → TA
2474 G → A
2667–2668 GA → deletion

Concentration

250–1,000 µg/ml

Chain Length

pHSG298: 2,675 bp
pHSG299: 2,673 bp

GenBank

Vector Name	Entry Name	Accession No.
pHSG298	SYNHSG299	M19415*
pHSG299	SYNHSG299	M19415

* Except for the MCS, pHSG298 has the same general sequence as pHSG299 with the following exception: pHSG298 lacks a G at position 1,446 in the sequence of pHSG299 that is registered with GenBank.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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