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pUC118 and pUC119 vectors

pUC118 and pUC119 are plasmid vectors developed for the preparation of single-stranded DNA. pUC118 and pUC119 were constructed by inserting the intergenic region (IG region) of the M13 phage DNA into the pUC18 and pUC19 plasmids, respectively. Infection by the M13K07 helper phage induces production of pUC118 and pUC119 as single-stranded DNA. Using this system, single-stranded DNA from large DNA fragments (up to 7 kb) can be stably obtained without deletion. Both pUC118 and pUC119 vectors contain multiple cloning sites (MCS).

Cat. #	Product	Size	Price
3319	pUC 119 DNA	25 ug	USD $76.00
pUC119 is a plasmid vector constructed by inserting a HgiA I(5645) - DraI(5941) fragment containing the intergenic region (IG region) of the M13 phage DNA into the NdeI site of the pUC19 plasmid. Since its multiple cloning site is in the lacZ region, any DNA inserted into this vector can be easily verified using plates containing IPTG and X-Gal. Moreover, the cloned DNA can be expressed using the lac promoter. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 3319: pUC 119 DNA
3318	pUC 118 DNA	25 ug	USD $76.00
pUC118 is a plasmid vector constructed by inserting a HgiA I (5645) - DraI (5941) fragment containing the intergenic region (IG region) of the M13 phage DNA into the NdeI site of the pUC18 plasmid. Since it has multi-cloning sites in the lacZ region, any DNA inserted into this vector can be easily verified using plates containing IPTG and X-Gal. Moreover, the cloned DNA can be expressed by using the lac promoter. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 3318: pUC118 DNA

Overview

Concentration: 0.5 µg/µl
Form: 10 mM Tris-HCI (pH8.0), 1 mM EDTA
Chain Length: 3,162 bp

More Information

Applications

Cloning of a target gene
DNA sequencing using M13 primers
Determination of long DNA sequences using Deletion Kit for Kilo-Sequencing (Cat. # 6030)
Gene expression using lac promoter

pUC118 DNA and pUC119 DNA

Each vector contains single cleavage sites for EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, PstI, SphI and HindIII upon restriction analysis

Storage

–20°C

GenBank

Vector	Entry name	Accession No.
pUC118	U07649	U07649
pUC119	U07650	U07650

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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