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M13mp18 RF phage vector

The M13mp18 RF phage vector is suitable for M13 sequencing by the dideoxy sequencing method. Since the phage vector contains a multiple cloning site in the lacZ region, recombinant vectors can be easily verified via blue/white colony screening using agar plates containing IPTG and X-Gal. This phage vector can also directly be used in M13 sequencing reactions because it is processed as a single-stranded DNA from phage particles.

Cat. #	Product	Size	Price	License	Quantity	Details
3118	M13 mp18 RF DNA	10 ug	USD $109.00
The M13 mp18 RF Phage Vector is suitable for M13 sequencing by the dideoxy sequencing method. Since the phage vector contains a multiple cloning site in the lacZ' region, recombinant vectors can be easily verified via blue/white colony screening using agar plates containing IPTG and X-Gal. The phage vector can also be directly used in M13 sequencing reactions because it is processed as a single-stranded DNA from phage particles. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back M13 mp18 RF DNA Vector Map M13 mp18 RF DNA Vector Map. Back 3118: M13 mp18 RF DNA

More Information

Storage

–20°C

Form

10 mM Tris-HCl (pH 8.0), 1 mM EDTA

GenBank

Entry name: M13MP18, Accession No.: X02513

Concentration

0.5 µg/µl

Note: Cloning of long DNA fragments (>4 kb) using the M13 mp18 RF phage vector can result in some colony deletions.

Product citations

Messing, J. New M13 vectors for cloning. Methods in Enzymology 101, 20–78 (1983).

Yanisch-Perron, C. et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33, 103–119 (1985).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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