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pUC18 and pUC19 vectors

The pUC18 and pUC19 plasmids enable successful cloning of large DNA fragments (larger than those cloned with a M13 mp18 RF Phage Vector). These cloning vectors contain a multiple cloning site at the lacZ' region that enables recombinant plamids to be verified via blue/white colony screening using agar plates containing IPTG and X-Gal. Expression of target DNA is enabled by the presence of a lac promoter in the cloning vectors. The pUC18 and pUC19 plasmids are suitable for dideoxy DNA sequencing using M13 primers. To sequence pUC18 and pUC19 plasmids containing large DNA insertions, utilize the Deletion Kit for Kilo-Sequencing (Cat. # 6030).

Cat. #	Product	Size	Price
3219	pUC 19 DNA	25 ug	USD $70.00
This DNA is a plasmid vector suitable for DNA sequencing by dideoxy method. It may allow successful cloning of DNA fragment longer than the case of M13 phage vector used. Since it has multi-cloning sites on lacZ' region, any DNA insertion into this vector can be easily verified using plates containing IPTG and X-Gal. Moreover, expression of the cloned DNA can be performed by using of lac promoter. M13 primers are available for DNA sequencing. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back 3219: pUC 19 DNA
3218	pUC 18 DNA	25 ug	USD $70.00
This DNA is a plasmid vector suitable for DNA sequencing by dideoxy method. This DNA may allow successful cloning of DNA fragment longer than the case of M13 phage vector used. Since it has multi-cloning sites on lacZ' region, any DNA insertion into this vector can be easily verified using plates containing IPTG and X-Gal. Moreover, expression of the cloned DNA can be performed by using of lac promoter. M13 primers are available for DNA sequencing. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back pUC18/19 Type Vector Map pUC18/19 Type Vector Map. Back 3218: pUC 18 DNA

More Information

Applications

Cloning and expression of target genes
DNA sequencing using M13 primers
Large DNA insert sequencing using Takara Bio's Deletion Kit for Kilo-Sequencing

Storage

–20°C

Concentration

250–1,000 µg/ml

Form

10 mM Tris-HCl (pH 8.0), 1 mM EDTA

Size

2,686 bp

GenBank

Vector	Entry Name	Accession No.
pUC18	SYNPUC18CV	L09136
pUC19	SYNPUC19CV	L09137

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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