Pyrithiamine pPTR vectors
The pyrithiamine or pPTR vectors are E. coli-Aspergillus shuttle vectors and contain an ampicillin resistance gene (AmpR) as the selection marker for E. coli and a pyrithiamine-resistance gene (ptrA) as the selection marker for Aspergillus. Pyrithiamine is a pyridine analog and inhibitor of thiamine. Because the pPTR vector multiple cloning site (MCS) is located within the lacZ region, the presence or absence of target DNA insert can easily be verified using blue/white screening via bacterial plates containing IPTG and X-Gal.
Overview
- pPTR I DNA (4,782 bp): chromosomal integration vector for Aspergillus sp.
- pPTR II DNA (10,031 bp): autonomously replicating vector for Aspergillus sp.
- These vectors are supplied in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA
More Information
Applications
Selection of Aspergillus sp. transformants using pyrithiamine
Unique restriction sites
- pPTR I DNA: HindIII, PstI, SmaI, and KpnI
- pPTR II DNA: HindIII, SmaI, and KpnI
Notes
Do not use reagents containing thiamine during transformation, as traces of thiamine will decrease the bacterial selection efficiency of pyrithiamine media.
Product citations
Kubodera, T., Yamashita, N. & Nishimura, A. Pyrithiamine resistance gene (ptrA) of Aspergillus oryzae: cloning, characterization and application as a dominant selectable marker for transformation. Biosci. Biotechnol. Biochem. 64, 1416–21 (2000).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2025 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
