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LA Taq DNA polymerase with GC buffers

TaKaRa LA Taq DNA Polymerase with GC Buffer has the Takara LA Taq DNA Polymerase blend for long and accurate PCR paired with GC-optimized buffers to power through difficult-to-amplify GC-rich sequences, including genomic DNA. The GC-optimized Buffers I and II are specifically designed to amplify GC-rich DNA templates (up to 73% GC content) or templates with a significant amount of secondary structure:

GC Buffer I is recommended for amplification of fragments that are ≥5 kb in length
GC Buffer II is recommended for fragments that are 2–3 kb in length

TaKaRa LA Taq DNA Polymerase with GC Buffer has the TaKaRa LA Taq DNA Polymerase blend for long-and-accurate PCR paired with GC-optimized buffers to power through difficult-to-amplify GC-rich sequences, including genomic DNA. The GC-optimized Buffers I and II are specifically designed to amplify GC-rich DNA templates (up to 73% GC content) or templates with a significant amount of secondary structure:

GC Buffer I is recommended for amplification of fragments that are ≥5 kb in length
GC Buffer II is recommended for fragments that are 2–3 kb in length

For the longest amplicons that are also GC rich, try PrimeSTAR GXL DNA Polymerase, which is designed to amplify genomic DNA up to 30 kb.

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Cat. #	Product	Size	Price	License	Quantity	Details
RR02AG	TaKaRa LA Taq® DNA Polymerase with GC Buffer	125 Units	USD $186.00
Cat. # RR02AG is Takara LA Taq DNA polymerase paired with two special GC-optimized Buffers. This system is designed to amplify templates with up to 73% GC content, and up to 20 kb in length routinely (extension up to 48 kb is possible on some templates). Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Resources Back Comparison of Takara LA Taq with GC Buffer and Takara LA Taq DNA polymerase for amplification of a GC-rich target fragment *Comparison of Takara LA Taq* with GC Buffer and Takara LA Taq DNA polymerase for amplification of a GC-rich target fragment.** A 2.1 kb mouse rRNA gene (63.4% GC) was amplified from of mouse genomic DNA. Takara LA Taq with GC Buffer resulted in high yield, high specificity amplification. Lanes: M1: lambda-Hind III digest 1 and 2: LA Taq / LA PCR Buffer II 3 and 4: LA Taq with GC Buffer / GC Buffer I 5 and 6: LA Taq with GC Buffer / GC Buffer II M2: pHY Marker . Back Amplification of the Huntington's Disease (HD) gene using Takara LA Taq DNA Polymerase *Amplification of the Huntington's Disease (HD) gene using Takara LA Taq* DNA Polymerase.** The HD gene is GC-rich and highly repetitive and the primers used in this experiment amplified regions of the HD gene IT15 CAG repeat. For all reactions, purified human genomic DNA (100 ng in a 50 µL reaction) was used as a template in reactions containing Takara LA Taq polymerase with either LA PCR Buffer II (lane 1), GC Buffer I (lane 2), or GC Buffer II (lane 3), or a competitor's high GC DNA polymerase kit (lane 4). The sizes of the amplified products were 262 bp (GC content 73%) and 358 bp (GC content 71.5%). Lane M contains a 100 bp ladder. Back RR02AG: TaKaRa LA Taq DNA Polymerase with GC Buffer

Overview

Long and accurate PCR amplification of genomic DNA targets (long-range PCR)
Optimized buffers (GC Buffers I & II) for amplification of templates with high GC content and/or significant secondary structure
High-yield amplification of long DNA targets with minimal optimization

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PCR products

PCR products generated with Takara LA Taq contain a mixture of 3'-A overhangs and blunt ends, which allows >80% cloning efficiency into T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low. It is also possible to clone the product in blunt-end vectors after blunting and phosphorylation of the end.

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

How the leopard lost its spots

Why do the offspring of some leopards hide their spots, resulting in black panthers? An international team of researchers focused on ASIP, a gene that causes dark coat coloration in other felines. Takara Bio's LA Taq with GC Buffer was used to sequence a tricky stretch of exon 4 in leopards and Asian golden cats, identifying SNPs associated with melanistic coat color. Scientists can now answer the curious question of how leopards produce panthers. That's Good Science!

Featured product: Takara LA Taq DNA Polymerase paired with GC-optimized buffers

See what our customers are saying about Takara LA Taq DNA Polymerase!

"It works very well for long pcr and easy optimise conditions. I have used it extensively in amplifiying 4kb to 17kb."
—Praveen Hajeri, UNIVERSITY OF MINNESOTA

"I have purchased your LA Taq. I tried it with and without adding pfu. I was looking for more product per PCR reaction. I got ~2X the amount vs my old Taq in a side by side test. Thanks "
—Maureen Tarsio, RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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LA Taq DNA polymerase with GC buffers

Notice to purchaser

Comparison of Takara LA Taq with GC Buffer and Takara LA Taq DNA polymerase for amplification of a GC-rich target fragment

Amplification of the Huntington's Disease (HD) gene using Takara LA Taq DNA Polymerase

RR02AG: TaKaRa LA Taq DNA Polymerase with GC Buffer