BcaBEST DNA Polymerase ver.2.0
BcaBEST DNA Polymerase ver.2.0 provides a robust, easy solution for rapid, point-of-care pathogen detection via loop-mediate isothermal amplification (LAMP), RT-LAMP, or other isothermal amplification techniques. Originating from the thermophilic bacteria Bacillus caldotenax, BcaBEST DNA Polymerase ver.2.0 has been engineered to enhance the activity of the DNA polymerase without compromising the enzyme’s strong strand-displacement activity, resulting in increased speed and sensitivity.
BcaBEST DNA Polymerase ver.2.0 provides a robust, easy solution for rapid, point-of-care pathogen detection via loop-mediate isothermal amplification (LAMP), RT-LAMP, or other isothermal amplification techniques. Originating from the thermophilic bacteria Bacillus caldotenax, BcaBEST DNA Polymerase ver.2.0 has been engineered to enhance the activity of the DNA polymerase without compromising the enzyme’s strong strand-displacement activity, resulting in increased speed and sensitivity.
Simply set up your reaction, incubate at 60–65°C for 30 min, and employ your detection method of choice to identify samples containing your desired nucleic acid sequence. BcaBEST DNA Polymerase ver.2.0 is compatible with fluorescent monitoring on a real-time PCR machine and various colorimetric/visual detection methods.
Overview
- Improved sensitivity compared to BcaBEST DNA Polymerase
- Reaction completes in 30 min or less
- High thermostability
- Compatible with real-time fluorescent monitoring and colorimetric/visual detection methods
BcaBEST DNA Polymerase ver.2.0 amplifies nucleic acids faster without sacrificing specificity
BcaBEST ver.2.0 |
Vendor N ver.2.0 | Vendor N ver.3.0 | Vendor Y | Vendor V | |
NTC | – | 44.35 | 24.25 | 35.5 | – |
10 pg | 16.6 | 31.9 | 20.8 | 33.85 | 57.4 |
100 pg | 14.9 | 28.1 | 13.85 | 28.25 | 55.9 |
Figure 1. Average Ct values obtained from LAMP reactions using commercially available DNA polymerases. Commercially available DNA polymerases that are advertised as appropriate for LAMP were used to amplify the fecA gene from JM109 genomic DNA, and reactions were monitored on a real-time PCR machine. Average Ct values for the no template control (NTC), reactions using 10 pg of genomic DNA, and reactions using 100 pg of genomic DNA are shown for each polymerase. LAMP reactions using BcaBEST ver.2.0 resulted in the lowest Ct values for the 10 pg and 100 pg reactions, which is indicative of the fastest reaction time, without amplification in the NTC reaction.
More Information
Applications
- LAMP, RT-LAMP, and other isothermal amplification techniques
- Rapid, point-of-care pathogen detection
- Low-resource field diagnostics
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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