Adenovirus (CAR) receptor booster
The CAR Receptor Booster renders cells that cannot usually be transduced with adenovirus susceptible to infection. It can also be used to increase adenoviral transduction efficiency in all cell types. The receptor-coated cell-derived nanovesicles combine with the plasma membrane of your target cells to temporarily elevate the density of the adenovirus receptor protein CAR on the cell surface.
The CAR Receptor Booster renders cells that cannot usually be transduced with adenovirus susceptible to infection. It can also be used to increase adenoviral transduction efficiency in all cell types. The receptor-coated cell-derived nanovesicles combine with the plasma membrane of your target cells to temporarily elevate the density of the adenovirus receptor protein CAR on the cell surface. For example, hematopoietic cells or CHO cells, which are usually resistant to adenoviral infection, can be transduced following a simple pretreatment with CAR Receptor Booster (see data images by expanding the Details arrow in the product table below).
How does it work?
The CAR Receptor Booster consists of a vial of concentrated cell-derived nanovesicles that are densely coated with the coxsackie virus and adenovirus receptor protein (CAR). When the booster vesicles are applied to your cells (10–20 µl per well of a 6-well plate for ~2 hr), they fuse with the plasma membrane, resulting in an increased level of the receptor on the cell surface. Your target cells can then be more efficiently transduced with recombinant adenovirus.
Overview
- Transduce any cell type with recombinant adenovirus, including hematopoietic cells and CHO cells which are known for having a low density of the CAR receptor
- Increase efficiency of transduction by temporarily increasing the concentration of the principal adenoviral receptor proteins on your target cells
- Simply preincubate your target cells with these concentrated exosome-like vesicles for 2 hr prior to infection with virus
- Increase the cell-surface density of the coxsackie and adenovirus receptor protein (CAR)
More Information
Applications
- Delivery of adenovirus to cells resistant to infection
Additional product information
Please see the produtct′s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Consistent data requires consistent titer
The Adeno-X qPCR Titration Kit combines qPCR with TB Green chemistry, allowing you to accurately determine the viral genome copy number in adenoviral preparations from a calibrated DNA standard curve.
See the data Viral transduction learning centerTakara Bio USA, Inc.
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