Advantage GC 2 polymerase mix & PCR kit
The Advantage GC 2 Polymerase Mix is specially formulated to amplify GC-rich templates. It includes the high-yield and high-fidelity Advantage 2 enzyme blend plus a proprietary GC-Melt Reagent and a reaction buffer containing DMSO—two additives proven to enable the amplification of templates with up to 90% GC content with minimal buffer optimization. The combination of DMSO and GC-Melt Reagent allow the amplification of virtually all GC-rich sequences that are resistant to PCR amplification by standard techniques.
For a complete kit, use the Advantage GC 2 PCR Kit (Cat. # 639120, 639119) which includes the enzyme blend, GC buffers, and dNTPs, plus GC-rich control template and primers.
The Advantage GC 2 Polymerase Mix and PCR kit are ideal for use with cDNA templates. For amplifying regions of GC-rich mammalian genomic DNA, we recommend using Advantage GC Genomic LA Polymerase Mix.
The Advantage GC 2 Polymerase Mix is specially formulated to amplify GC-rich templates. It includes the high-yield and high-fidelity Advantage 2 enzyme blend plus a proprietary GC-Melt Reagent and a reaction buffer containing DMSO—two additives proven to enable the amplification of templates with up to 90% GC content with minimal buffer optimization. The combination of DMSO and GC-Melt Reagent allow the amplification of virtually all GC-rich sequences that are resistant to PCR amplification by standard techniques.
For a complete kit, use the Advantage GC 2 PCR Kit (Cat. # 639120, 639119) which includes the enzyme blend, GC buffers, and dNTPs, plus GC-rich control template and primers.
The Advantage GC 2 polymerase mix and PCR kit are ideal for use with cDNA templates. For amplifying regions of GC-rich mammalian genomic DNA, we recommend using Advantage GC Genomic LA Polymerase Mix.
If you are interested in Commercial Use of our products, including OEM, bulk purchasing, or custom formulation (e.g., highly concentrated reagents, custom volumes), please contact Business Development at oem@takarabio.com to discuss your needs, or visit our OEM page to submit an inquiry.
Overview
- Optimized for use with complex, GC-rich cDNA and genomic DNA templates
- Generates error-free PCR products
- Amplifies targets of up to 6 kb
More Information
Applications
- GC-rich PCR
- Long and accurate PCR
- Hot-start PCR
- Difficult-target PCR
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
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There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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