Myc-tag and HA-tag vectors: mammalian expression vectors
These mammalian expression vectors allow you to express a protein of interest fused to either the c-Myc or hemagglutinin (HA) epitope tag. c-Myc and HA are well-characterized and highly immunoreactive tags, and thus are ideal for co-IP studies but are also easily detected via Western blot. Moreover, c-Myc and HA are small tags and thus unlikely to interfere with the function of your protein.
These mammalian expression vectors allow you to express a protein of interest fused to either the c-Myc or hemagglutinin (HA) epitope tag. c-Myc and HA are well-characterized and highly immunoreactive tags, and thus are ideal for co-IP studies but are also easily detected via Western blot. Moreover, c-Myc and HA are small tags and thus unlikely to interfere with the function of your protein.
The myc- or HA-tagged protein of interest can be detected by either western analysis or fluorescence microscopy using an anti-myc antibody or anti-HA antibody.
The pCMV-Myc & pCMV-HA Vector Set includes c-Myc Monoclonal and HA-Tag Polyclonal Antibodies, which can also be used in Western blot, ELISA, or immunostaining assays. A sequencing primer is also included in the set to confirm that the insert is in the correct reading frame.
HA tag sequence: ACC ATG TAC CCA TAC GAT GTT CCA GAT TAC GCT
Myc tag sequence: ATG GCA TCA ATG CAG AAG CTG ATC TCA GAG GAG GAC CTG
Overview
- Co-immunoprecipitation of myc-tagged and HA-tagged proteins in mammalian cells
- Strong constitutive expression
- Confirm yeast two-hybrid interactions in mammalian cells
- Strong, clean bands via Western blot
More Information
Applications
- Co-immunoprecipitation studies
- Confirmation of protein-protein interactions
- Western blotting
- Epitope-tagged expression
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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