Analysis of glycosphingolipids: recombinant endoglycoceramidase II (rEGCase II)

Recombinant endoglycoceramidase II (rEGCase II) is an endo-type enzyme that cleaves the linkages between oligosaccharides and ceramides of acidic and neutral glycosphingolipids, producing intact oligosaccharide and ceramide byproducts. This enzyme does not act on galactosyl- or glucosylceramides. It also does not hydrolyze cerebrosides, sulfatides, sphingomyelin, glycoglycerolipids, or glycoproteins. Globo-type glycosphingolipids are highly resistant to rEGCase II hydrolysis. rEGCase II is most suited to the structural analysis of glycosphingolipids.

Cat. #	Product	Size	Price	License	Quantity	Details
4460	rEGCase II	100 mU	USD $455.00
Endoglycoceramidase II (EGCase II) cleaves the linkage between the oligosaccharide and ceramide of various acidic and neutral glycosphingolipids, producing intact oligosaccharides and ceramides. This enzyme does not act on galactosyl- and glucosyl-ceramides, glycoglycerolipids, or glycoproteins. Note: This product contains detergents and is not active without them. Do not use on living cells. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 4460: rEGCase II

More Information

Applications

Structural analysis of glycosphingolipids

Source

Recombinant E. coli

Purity

rEGCase is free from the following exoglycosidase activities: alpha-galactosidase, beta-galactosidase, alpha-N-acetylgalactosaminidase, beta-N-acetylgalactosaminidase, beta-N-acetyl-glucosaminidase, alpha-mannosidase, alpha-fucosidase, and sialidase. This enzyme is also free from glycopeptidase, endo-beta-N-acetyglucosaminidase, proteinase, and sphingomyelinase activities.

Storage

Store at –20°C until use. Thawed solution is stable for 2–3 days at 4°C.

Note

This product contains detergents and requires them for optimal activity. Do not use on living cells.

Properties

Systematic name: Oligoglycosylglucosylceramide glycohydrolase (EC 3.2.1.123)
Form: 20 mM sodium acetate solution (pH 6.0), 0.2% BSA, 0.1% Lubrol PX
Concentration: 100 mU/100 µl
Optimum pH: 5.0–6.0
Inhibitors: 1 mM Hg²⁺, Zn²⁺, Cu²⁺
Not inhibited by Ba²⁺, Ca²⁺, Mg²⁺, or EDTA

References

Ito, M. & Yamagata, T. Purification and characterization of glycosphingolipid-specific endoglycosidases (endoglycoceramidases) from a mutant strain of Rhodococcus sp. Evidence for three molecular species of endoglycoceramidase with different specificities. J. Biol. Chem. 264, 9510–9519 (1989).

Park, J.T. & Johnson, M.J. A submicrodetemination of glucose. J. Biol. Chem. 181, 149–151 (1949).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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