Westase Enzyme

Westase enzyme enables fungal and yeast cell wall digestion via lysis of beta-glucanase (beta-1,6 glucanase and beta-1,3-glucanase) bonds for experimental protocols including spheroplast generation, immunofluorescence, protein purification, and transformation. Westase Enzyme works well for beta-glucanase lysis in Ascosporogenous-type yeasts such as Saccharomyces cerevisiae. It can also be used for yeast cell wall digestion of fission yeasts such as Schizosaccharomyces pombe and Basidiosporogenous since they cannot be protoplasted with simple Zymolyase treatment.

Cat. #	Product	Size	Price	License	Quantity	Details
9005	Westase™ Enzyme	1 g	USD $197.00
Westase Enzyme possesses lytic yeast cell walls. Its primary enzyme activities consist of β-1,6 glucanase and β-1,3 glucanase activity. This enzyme works well for ascosporogenous yeasts such as Saccharomyces cerevisiae. It can also be used with fission yeasts such as Schizosaccharomyces pombe, which cannot be protoplasted with Zymolyase treatment, or basidiosporogenous yeasts. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components

More Information

Applications

Yeast cell wall digestion
Beta-glucanase bond lysis

Source

Westase Enzyme is prepared from Streptomyces rochei DB-34 liquid cell culture supernatant.

Storage

4°C (dry condition)

Notes

Manufactured by Ozeki Corporation.

Form

Lyophilized powder (containing celite as the excipient).

Definition of activity

Beta-1,6 glucanase activity: One unit is defined as the amount of enzyme required to release 1 µM of reduced sugar from 10 mg/ml Pustulan solution in 1 min. at 37°C (pH 6.0).

Lytic activity: One unit is defined as the amount of enzyme required to decrease abosrbance at 660 nm by 1% in 1 min. at 30°C (pH 6.0) when using Cryptococcus albidus IFO 0612 cell wall fraction as a substrate.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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