GST-tagged protein purification kit
Glutathione-Superflow allows rapid affinity purification of GST-tagged proteins for batch, gravity-flow, and FPLC applications. The resin is based on 6% cross-linked agarose, with glutathione covalently bound to the resin. It can withstand flow rates as high as 15 ml/cm2/min and medium pressure (up to 150 psi).
The GST Purification Kit contains sufficient buffers and Glutathione-Superflow Columns for performing five batch/gravity-flow purifications. Up to 10 mg per prep of GST-tagged recombinant proteins can be purified using this kit.
Glutathione-Superflow allows rapid affinity purification of GST-tagged proteins for batch, gravity-flow, and FPLC applications. The resin is based on 6% cross-linked agarose, with glutathione covalently bound to the resin. It can withstand flow rates as high as 15 ml/cm2/min and medium pressure (up to 150 psi).
The GST Purification Kit contains sufficient buffers and Glutathione-Superflow Columns for performing five batch/gravity-flow purifications. Up to 10 mg per prep of GST-tagged recombinant proteins can be purified using this kit.
GST tags
Epitope tags such as glutathione-S-transferase (GST) are often used as tags for proteins for expression and purification applications. Glutathione transferases are abundant enzymes involved in cellular defense against electrophilic chemical compounds, which bind glutathione with high affinity and specificity. The strength and selectivity of this interaction can be exploited to effectively purify GST-tagged proteins. Glutathione-Superflow Resin selectively binds the GST-tagged protein under normal conditions, allowing the protein of interest to be separated from whole-cell extracts rapidly and efficiently. A high degree of purification can be achieved in just one chromatographic step.
GST is a 35-kDa protein that can be assayed biochemically as well as immunologically. This characteristic sets it apart from many epitope tags that are simply short peptides. However, since GST is a large tag, the potential for degradation by proteases is higher than for smaller tags. Therefore, performing GST-based protein purification as quickly as possible under nondegrading conditions is necessary to minimize sample loss. GST loses its ability to bind Glutathione-Superflow Resin when denatured, and consequently strong denaturants such as guanidine-HCl or urea must not be used in the purification buffers. Check reagent compatibilities when designing your purification scheme.
Overview
- Easy to use—no optimization required
- Purifies more protein—high binding capacity (>10 mg of recombinant GST fusion protein per ml of resin)
- Yields highly purified protein—eluted protein is free of cellular contaminants
- Reusable—easy protocol for regenerating and storing resin
- Save money—30–40% less expensive than competing brands
More Information
Applications
- Purification of GST-tagged proteins: batch, gravity-flow, or FPLC applications
Reagents compatible with Glutathione-Superflow Resin
| Reagent | Acceptable Concentration |
| Beta-mercaptoethanol | 10 mM (with caution) |
| EDTA, EGTA | 10 mM |
| Ethanol | 30%, for regeneration only |
| Glycerol | 20% |
| HEPES | 50 mM |
| Imidazole | 200 mM at pH 7.0–8.0 |
| KCl | 500 mM |
| MES | 20 mM |
| MOPS | 50 mM |
| NaCl | 1.0 M |
| NP-40 | 1% |
| Tris | 50 mM |
Reagents incompatible with Glutathione-Superflow Resin
These reagents are incompatible at any concentration:
- CHAPS
- DTE (dithioerythritol) and DTT (dithiothreitol)
- Guanidine hydrochloride
- SDS
- Urea
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2024 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
