T4 DNA Ligase

T4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in double-stranded DNA using ATP as a coenzyme. Both blunt and cohesive end DNA ligation, as well as single-stranded nick repair of DNA, RNA and DNA/RNA, are possible using the T4 DNA ligase.

Cat. #	Product	Size	Price
2011B	T4 DNA Ligase	125,000 Units	USD $259.00
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between double-stranded DNA fragments with 3'-OH and 5'-phosphate ends, in the presence of ATP. T4 DNA Ligase is supplied in 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mm DTT, 0.1 mM EDTA and 50% glycerol. Cat. # 2011B contains 5 of Cat. # 2011A. Please refer to Cat. # 2011A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 2011B: T4 DNA Ligase
2011A	T4 DNA Ligase	25,000 Units	USD $67.00
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between double-stranded DNA fragments with 3'-OH and 5'-phosphate ends, in the presence of ATP. T4 DNA Ligase is supplied in 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mm DTT, 0.1 mM EDTA and 50% glycerol. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 2011A: T4 DNA Ligase

More Information

Applications

Blunt and cohesive end DNA ligation
Blunt end linker addition
Single-strand DNA, RNA and DNA/RNA repair
DNA fragment insertion into vectors

Source

Recombinant E. coli

Storage

–20°C

Unit definition

One ligation unit is defined as the amount of enzyme that ligates >90% of 6 µg of Hind III-restricted lambda DNA in a 20 µl reaction volume in 30 minutes at 16°C. One unit of the enzyme corresponds to 0.008 Weiss units by the ATP-PP_i exchange reaction in 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl₂, 10 mM DTT, 66 µM ATP and 3.3 µM [³²P]Na₄P₂O₇.

Concentration

350 U/µl (2.8 Weiss units/µl)

Product citations

Weiss, B., Jacquemin-Sablon, A., Live, T. R., Fareed, G. C. & Richardson, C. C. Enzymatic breakage and joining of deoxyribonucleic acid. VI. Further purification and properties of polynucleotide ligase from Escherichia coli infected with bacteriophage T4. J. Biol. Chem. 243, 4543–55 (1968).

Weiss, B., Thompson, A. & Richardson, C. C. Enzymatic breakage and joining of deoxyribonucleic acid. VII. Properties of the enzyme-adenylate intermediate in the polynucleotide ligase reaction. J. Biol. Chem. 243, 4556–63 (1968).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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