DNA Ligation Kit, Mighty Mix

The DNA Ligation Kit, Mighty Mix is a 2X ligation premix for high-efficiency ligations, especially for blunt-ended ligations. This DNA ligation kit can also be used for ligation into TA-vectors. The 2X ligation mix is compatible with small ligation reaction volumes (10 µl) for when DNA quantity is a limiting factor, and it contains sufficient reagent for 75–150 reactions. The reaction mixtures from this DNA ligation kit can be used directly in bacterial transformation.

Cat. #	Product	Size	Price	License	Quantity	Details
6023	DNA Ligation Kit, Mighty Mix	100 Rxns	USD $243.00
Takara's DNA Ligation Kit, Mighty Mix, is a one-solution premix ligation reagent that offers high efficiency ligations, particularly for blunt-ended ligation. The reaction can be performed for 5 minutes at 25°C (rapid protocol), or 30 minutes at 16°C (standard protocol) with equal results for both circular and linear DNA ligations. The 2X Ligation Mix allows small ligation reaction volumes (10 μl) when DNA is limiting. The kit includes sufficient reagent for 100 ligation reactions. Ligation products reaction can be used directly in bacterial transformation. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Resources Back Blunt-end ligation efficiency with Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors Blunt-end ligation efficiency with Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors. Each ligation reaction contained 25 fmol of BAP-treated pUC118-Hinc II vector and 75 fmol of a 500 bp insert. Ligation efficiency was assessed by blue/white colony screening. Back TA-vector ligation efficiency of Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors TA-vector ligation efficiency of Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors. Each ligation reaction contained 13 fmol of a pT7 Blue T-Vector and 39 fmol of a 2.08 kb PCR Product. Ligation efficiency was assessed by blue/white colony screening. Back Recommended reaction conditions Recommended reaction conditions. Note: This kit retains activity through 50 freeze-thaw cycles. The Ligation Mix should be thawed on ice (5-10 minutes) and mixed well by pipetting prior to use. . Back 6023: DNA Ligation Kit, Mighty Mix

Overview

Enables high-efficiency ligations for cohesive, blunt-end, and TA cloning
2X ligation mix adds convenience and eliminates extra pipetting and purification steps
Ligation reactions can be quickly performed at either 5 or 30 minutes depending on DNA type
Reaction volumes are scalable

More Information

Applications

Highly efficient and fast DNA ligation
Small reaction volume ligation
TA and blunt-end cloning

Product citation

Hayashi, K., Nakazawa, M., Ishizaki, Y., Hiraoka, N. & Obayashi, A. Regulation of inter- and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol. Nucleic Acids Res. 14, 7617–31 (1986).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab in the product table.

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DNA Ligation Kit, Mighty Mix

Notice to purchaser

Blunt-end ligation efficiency with Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors

TA-vector ligation efficiency of Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors

Recommended reaction conditions

6023: DNA Ligation Kit, Mighty Mix

Overview

More Information

Applications

Product citation

Additional product information