Glycobiology enzymes: Alpha 1, 3/4-L Fucosidase

Alpha 1, 3/4-L Fucosidase (systemic name: Alpha-L-Fucoside Fucohydrolase) is a useful glycobiology enzyme and specifically cleaves alpha 1,3 and alpha 1,4 fucoside bonds, liberating L-fucose from lacto-N-fucopentaose II, lacto-N-fucopentaose III, and N-acetyllactosamine type tri-antennary and tetra-antennary sugar chains containing alpha 1,3 fucoside bonds. It also liberates L-fucose from alpha 1-acid glycoproteins.

Cat. #	Product	Size	Price	License	Quantity	Details
4453	Alpha 1, 3/4-L-Fucosidase	100 uU	USD $485.00
This enzyme catalyses the hydrolytic cleavage of α-fucoside bonds and releases L-fucose. This enzyme specifically cleaves α1, 3- and α1, 4-fucoside bonds. It also releases L-fucose from α1-acid glycoprotein. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Substrate specificity for alpha 1,3/1,4 fucosidase (Cat.# 4453) Substrate specificity for alpha 1,3/1,4 fucosidase (Cat.# 4453). Back 4453: Alpha-1,3/4-L-Fucosidase

More Information

Applications

Specifically cleaves alpha1,3 and alpha1,4 fucoside bonds
Liberates L-fucose from lacto-N-fucopentaose II, lacto-N-fucopentaose III and N-acetyllactosamine type tri-antennary and tetra-antennary sugar chain with alpha1,3 fucoside bonds
Also liberates L-fucose from alpha1-acid glycoprotein

Source

Streptomyces sp. 142

Storage

Store at –20°C. The stock solution is stable for at least 12 months at 4°C. Avoid repeated freeze-thaw cycles.

Properties

Systematic name: Alpha-L-Fucoside fucohydrolase (E.C. 3.2.1.51)
Molecular weight: 40 kDa
Concentration: 1 mU/ml
K_m (substrate):
- Substrate 1: 10.1 µM (PA-lacto-N-fucopentaose II)
- Substrate 2: 1.08 µM (PA-lacto-N-fucopentaose III)
Optimum pH: pH 6.0 (Substrate 2)
Range of pH stability: pH 4.5–7.0 (37°C, 60 min.)
Stabilizer: 0.1% NP-40, 0.1% Brij-58, and 0.02% BSA

Form

In a solution of 50 mM potassium phosphate buffer (pH 6.0) containing 0.1 M NaCl, 0.02% NaN₃ and 0.1% Brij-58

Product citations

Sano, M., Hayakawa, K. & Kato, I. Purification and characterization of alpha-L-fucosidase from Streptomyces species. J. Biol. Chem. 267, 1522–7 (1992).

Definition of activity

One unit is defined as the amount of enzyme required to hydrolyze 1 µmol of pyridylamino-lacto-N-fucopentaose III in 1 minute at 37°C and pH 6.0.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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