Isolation of genomic DNA from bacteria, yeast, and fungi—NucleoSpin Microbial DNA
The NucleoSpin Microbial DNA kit is designed for rapid purification of highly pure genomic DNA from microorganisms (gram-negative and gram-positive bacteria, yeast, and fungi). The kit utilizes MN Bead Tubes Type B (glass beads; included in the kit) in combination with Liquid Proteinase K and a common disruption device, such as an MN Bead Tube Holder or a swing mill, to lyse different sample types using a single procedure. The procedure is fast, simple and provides reliably high DNA yields from a wide range of microbial samples. MN bead tubes can also be ordered separately if needed.
The NucleoSpin Microbial DNA kit is designed for rapid purification of highly pure genomic DNA from microorganisms (gram-negative and gram-positive bacteria, yeast, and fungi). The kit utilizes MN Bead Tubes Type B (glass beads; included in the kit) in combination with Liquid Proteinase K and a common disruption device, such as an MN Bead Tube Holder or a swing mill, to lyse different sample types using a single procedure. The procedure is fast, simple and provides reliably high DNA yields from a wide range of microbial samples. MN bead tubes can also be ordered separately if needed.
Appropriate conditions for DNA binding to NucleoSpin Microbial DNA Columns are achieved by addition of large amounts of chaotropic salts (Binding Buffer MG) to the lysate. Contaminants are removed by two efficient washing steps. The DNA is then eluted with a slightly alkaline buffer and is ready to use.
Overview
- Suitable for a wide variety of starting materials
- Includes MN bead tubes for efficient lysis—compatible with many cell disruption devices
- Fast and easy procedure—Liquid Proteinase K included; no additional enzyme required
More Information
| Technology | Silica-membrane technology combined with MN Bead Tubes Type B |
| Format | Mini spin columns |
| Starting material | <40 mg wet weight cell pellet |
| Fragment size | 200 bp–approximately 50 kb |
| Typical yield | Depends on sample type and disruption method Approximately 5–25 µg (30 mg wet weight cell pellet) |
| A260/A280 |
1.6–2.0 |
| Elution volume | 100–200 µl |
| Preparation time | 35 min/prep |
| Binding capacity | 60 µg |
Applications
- Total DNA from microbial cultures
- Typical downstream applications: PCR, real-time PCR, southern blotting, enzymatic reactions
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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