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Technical notes View Capturem Streptavidin data

Capturem Streptavidin

Capturem Streptavidin mini columns and 96-well plate

Capturem Streptavidin mini columns and 96-well plates take advantage of immobilized streptavidin on high-capacity Capturem membranes to enable rapid, high-quality purification or enrichment of biotinylated targets. Alternatively, biotinylated ligands bound to Capturem Streptavidin membranes can be used to pull down target molecules or complexes.

Capturem Streptavidin mini columns and 96-well plates take advantage of immobilized streptavidin on high-capacity Capturem membranes to enable rapid, high-quality purification or enrichment of biotinylated targets. Alternatively, biotinylated ligands bound to Capturem Streptavidin membranes can be used to pull down target molecules or complexes.

Streptavidin is a cell wall protein from the bacterium Streptomyces avidinii with a high affinity for biotin, and this interaction has found extensive use in the life sciences for enrichment and other applications. Capturem kits use a novel high-capacity membrane-based affinity technology that allows the workflows to be completed in just minutes at room temperature, from loading of samples to elution.

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Cat. # Product Size Price License Quantity Details
635733 Capturem™ Streptavidin Miniprep Columns 20 Columns USD $429.00

Capturem Streptavidin Miniprep Columns are single-use, disposable columns for simple, rapid enrichment of target proteins and antibodies that bind biotinylated proteins. The membranes are guaranteed to bind more than 15 μg of biotinylated BSA control per column. These miniprep columns are suitable for enrichment of target proteins and antibodies, including those from animal serum, cell culture lysates (e.g., mammalian or bacterial cell lysates), and cell culture supernatants of hybridoma cell lines. Each column can hold up to 850 µl of sample and requires a minimum elution volume of 100 µl.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data
635734 Capturem™ Streptavidin 96-Well Plate 1 x 96-well plate USD $804.00

The Capturem Streptavidin 96-Well Plate is a single-use, disposable plate for simple, rapid enrichment of target proteins and antibodies that bind biotinylated protein. The plate binds more than 15 µg of biotinylated BSA control per well. This plate is suitable for enrichment of target proteins and antibodies, including those from animal serum, cell culture lysates (e.g., mammalian or bacterial cell lysates), and cell culture supernatants of hybridoma cell lines. Each well can hold up to 1 ml of sample and requires a minimum elution volume of 50 µl.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Overview

  • Simple, ultra-fast workflow: 5–15-minute room-temperature protocols
  • High concentration: extremely small elution volumes yield highly concentrated complexes
  • High quality: short residence times reduce the possibility of aggregation, complex dissociation, or loss of activity
  • High reproducibility: consistent results from well to well
  • Single use: novel membrane technology assembled into disposable spin columns reduces the risk of contamination and carryover

More Information

Applications

  • Purification or enrichment of biotinylated proteins
  • Pull-down of binding partners (e.g., proteins or oligos) for a biotin-labeled ligand

Loading capacities and times for available Capturem Streptavidin formats

Biotin-antibody Biotin-BSA Biotin-ssDNA Free biotin Purification time
Capturem Streptavidin miniprep columns and 96-well plates 20–40 µg >15 µg 1,000–1,500 ng >4,000 pmol 15 minutes

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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