TaKaRa BCA Protein Assay Kit
The bicinchoninic acid (BCA) protein assay is a technique used to accurately quantify protein concentration from biological material. The TaKaRa BCA Protein Assay Kit is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions and is based on a two-step reaction in one tube. The BCA protein assay is not affected by the type of protein, exhibits linearity across a wide range of protein concentrations (0.02–2 mg/ml), and is resistant to the effects of surfactants.
Overview
- Detect down to 0.02 mg/ml of protein with a linearity range of 0.02-2 mg/ml
- Quantify protein concentration regardless of protein composition or presence of detergents
More Information
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Technical notes
Purification of active, secreted proteins
Purify active, secreted his-tagged proteins from cell-culture supernatants in 15 minutes.
A revolutionary solution for protein purification
Learn about a novel solution for recombinant protein purification from mammalian and bacterial cell lysates.
Capturem large volume
A simple, rapid method for purifying and concentrating his-tagged protein from large sample volumes.
Magnetic beads for protein purification
Magnetic beads enable one-step protein purification and pull-down assays.
Obtain highest purity with cobalt resin
Prepare exceptionally pure his-tagged proteins from bacterial, mammalian, yeast, and baculovirus-infected cells.
Purification tips and tricks
How to choose the right his-tagged purification resin
Learn how to decide between nickel- and cobalt-based IMAC resins for your his-tagged purification.
When your his-tagged constructs don’t bind—troubleshooting your protein purification woes
This blog post covers reasons his-tag fusions might not bind to resin and how to tackle these issues.
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