Purified, recombinant fluorescent proteins—GFP, DsRed

These Living Colors recombinant proteins are purified from E. coli. Each recombinant variant has absorption and emission spectra identical to those for the expressed proteins. The purified protein retains its fluorescence capability under many harsh conditions, including high heat, pH extremes, exposure to chaotropes, and incubation with low concentrations of detergents. These protein are suitable as standards in Western blots and protein gels.

These Living Colors recombinant proteins are purified from E. coli. Each recombinant variant has absorption and emission spectra identical to those for the expressed proteins. The purified protein retains its fluorescence capability under many harsh conditions, including high heat, pH extremes, exposure to chaotropes, and incubation with low concentrations of detergents. These protein are suitable as standards in Western blots and protein gels. They can also be used as calibration standards for fluorometers and flow cytometry instruments.

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Cat. #

Product

Size

Price

License

Quantity

Details

632502

rAcGFP1 Protein

100 ug

USD $443.00

License Statement

ID Number
39	AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

Affinity purified recombinant Aequorea coerulescens green fluorescent protein (rAcGFP1) can be used as a standard for protein gels, Western blots, and fluorometry. It can also be used as a control for expression studies and for microinjection into cells and tissues. At its N-terminus, rAcGFP1 contains a 6xHN epitope tag, used for purification of the protein by immobilized metal affinity chromatography (IMAC) with our TALON Resin. An enterokinase (EK) site, located between the tag and rAcGFP1, makes it possible to remove the 6xHN polypeptide if desired. The 6xHN tag does not interfere with the protein's characteristic spectral properties.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

632437

rDsRed-Express Protein

100 ug

USD $443.00

License Statement

ID Number
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.

Affinity purified recombinant Discosoma sp. red fluorescent protein (rDsRed-Express). rDsRed-Express can be used as a standard for protein gels, Western blots, fluorometry, and fluorescence activated cell sorting (FACS). It can also be used as a control for DsRed-Express expression studies and for microinjection into cells and tissues. At its N-terminus, rDsRed-Express contains a 6xHN epitope tag, used for purification of the protein by metal ion affinity chromatagraphy (IMAC) with our TALON Resin. An enterokinase (EK) site, located between the tag and DsRed-Express, makes it possible to remove the 6xHN polypetide if desired. The 6xHN tag does not interfere with the protein's characteristic spectral properties.

Notice to purchaser

Components

Overview

Purified, recombinant proteins from E. coli
Protein retains the absorption and emission spectra identical to expressed proteins
Retains fluorescence capability under harsh conditions

More Information

Applications

Western blotting
Calibration of fluorescent protein detection instrumentation
Microinjection in cells and tissues

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Fluorescent protein vector finder

Takara Bio offers hundreds of different fluorescent protein vectors for various applications. To help you select a fluorescent protein vector, we have created a tool that filters vectors based on the color of the fluorescent protein expressed, vector name, or suitable applications.

Try the tool Fluorescent protein quick guide

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