MicroRNA expression vectors
These vectors provide strong miRNA expression and allow you to verify and track that expression with a fluorescent protein marker. The pmR-mCherry and pmR-ZsGreen1 Vectors couple your miRNA expression cassette to a bright red or green Living Colors fluorescent reporter, for miRNA expression you can see and select.
These vectors provide strong miRNA expression and allow you to verify and track that expression with a fluorescent protein marker. The pmR-mCherry and pmR-ZsGreen1 Vectors couple your miRNA expression cassette to a bright red or green Living Colors fluorescent reporter, for miRNA expression you can see and select.
How pmR miRNA expression vectors work
Clone your miRNA sequence into the vector’s multiple cloning site (MCS) located in the 3' untranslated region (UTR) of the fluorescent protein’s mRNA transcript. This enables both molecules to be expressed simultaneously from the vector’s strong CMV promoter.
Each vector is equipped with a powerful CMV promoter, a selectable marker, and a fluorescent protein expression cassette containing either mCherry or ZsGreen1—two of our brightest Living Colors fluorescent proteins. With these vectors, you can clone and express your favorite miRNA, and then select, sort, and/or visualize the cells in which it is expressed.
Overview
- Strong coexpression of miRNA and a bright fluorescent protein
- Clone your miRNA into the 3' UTR of mCherry or ZsGreen1
- Simultaneously verify miRNA expression and transfection efficiency via fluorescent microscopy
More Information
Applications
- Over-expression of microRNAs to study gene or microRNA function
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2024 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
