iDimerize antibodies

Antibodies against iDimerize Homodimer DmrB DmrA and DmrC domains. Also detect FKBP12 and FRB fusions

After fusing your protein of interest to one to the dimerization tags provided with iDimerize Homodimerization Systems (DmrB) or iDimerize Heterodimerization Systems (DmrA and DmrC), you may wish to monitor the protein's expression by SDS-PAGE followed by Western blot. These sensitive antibodies give a clean band on a Western blot. The DmrA and DmrB antibodies are monoclonal mouse antibodies, and the anti-DmrC antibody is polyclonal, derived from rabbit.

Cat. #	Product	Size	Price
635091	DmrC Polyclonal Antibody	100 ug	USD $467.00
The DmrC Polyclonal Antibody was raised in rabbit and recognizes as little as 20–40 ng of DmrC-tagged fusion protein expressed using any iDimerize Inducible Heterodimer System. The antibodies are also able to detect fusions created using ARGENT regulation systems: DmrA is identical to wild type FKBP12, DmrB is identical to the F36V mutant of FKBP12, DmrC is identical to the T82L mutant of FRB. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Western blot detection of a DmrC-tagged protein with the DmrC Polyclonal Antibody (Cat. No. 635091) Western blot detection of a DmrC-tagged protein with the DmrC Polyclonal Antibody (Cat. No. 635091). HeLa cells were transfected with a plasmid engineered to express a DmrC-AcGFP1 fusion protein. Lysate was prepared from the equivalent of 3.5 x 10⁴ cells and resolved on a polyacrylamide gel, followed by Western blot analysis with the DmrC Polyclonal Antibody (1:1000 dilution). A single band was detected at the expected molecular weight (Lane 3). No band was detected in lysate from untransfected HeLa cells (Lane 1) or HEK 293 cells (Lane 2). Back The DmrC Polyclonal Antibody (Cat. No. 635091) can detect as little as 16 ng of DmrC protein The DmrC Polyclonal Antibody (Cat. No. 635091) can detect as little as 16 ng of DmrC protein. The indicated amounts of recombinant DmrC protein (12 kDa) were resolved by SDS-PAGE and subjected to Western blot analysis. The DmrC Polyclonal Antibody was able to detect less than 16 ng of purified protein. Lysate from HEK 293 cells was used as a negative control (Lane 1).
635090	DmrB Monoclonal Antibody	100 ug	USD $467.00
The DmrB Monoclonal Antibody recognizes DmrB-tagged fusion proteins expressed using any iDimerize Inducible Homodimer System. The isotype of this mouse monoclonal antibody is IgG1 Kappa. The antibodies are also able to detect fusions created using ARGENT regulation systems: DmrA is identical to wild type FKBP12, DmrB is identical to the F36V mutant of FKBP12, DmrC is identical to the T82L mutant of FRB. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Western blot detection of a DmrB-tagged protein using the DmrB Monoclonal Antibody (Cat.No. 635090) Western blot detection of a DmrB-tagged protein using the DmrB Monoclonal Antibody (Cat. # 635090). HEK 293 cells were transfected with the pAcGFP1-DmrB plasmid. Lysate from the equivalent of 3.5 x 10⁴ cells was run on a polyacrylamide gel (Lanes 2 and 3) and analyzed by Western blot using the DmrB Monoclonal Antibody (1:1000 dilution). A predominant band was detected at the expected molecular weight. The band was detected in lysate from cells treated with (Lane 3) or without (Lane 2) B/B Homodimerizer, but not in lysate from untransfected HEK 293 cells (Lane 1). Back 635090: DmrB Monoclonal Antibody
635089	DmrA Monoclonal Antibody	100 ug	USD $467.00
The DmrA Monoclonal Antibody recognizes DmrA-tagged fusion proteins expressed using any iDimerize Inducible Heterodimer System. The isotype of this mouse monoclonal antibody is IgG1 Kappa. The antibodies are also able to detect fusions created using ARGENT regulation systems: DmrA is identical to wild type FKBP12, DmrB is identical to the F36V mutant of FKBP12, DmrC is identical to the T82L mutant of FRB. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Western blot detection of a DmrA-tagged protein using the DmrA Monoclonal Antibody (Cat. No. 635089) Western blot detection of a DmrA-tagged protein using the DmrA Monoclonal Antibody (Cat. No. 635089). HEK 293 cells were transfected with a plasmid expressing a DmrA-tagged CherryPicker fusion protein. The cell lysate was resolved by SDS-PAGE and subjected to Western blot analysis with the DmrA Monoclonal Antibody. A predominant band was detected in transfected HEK 293 cells (Lane 2), but no signal was detected in the untransfected control cell lysate (Lane 1). Back 635089: DmrA Monoclonal Antibody

Overview

The DmrB and DmrA domains are highly homologous, so the antibodies cross-react with each others' domains
The anti-DmrC antibody does not cross-react with the other domains
Detection of DmrB domains used for iDimerize Homodimer Systems
Detection of DmrA and DmrC domains used for iDimerize Heterodimer Systems
DmrB antibody can be used for detection of DmrD domains used for the Reverse Dimerization Systems
DmrA is identical to wild type FKBP12
DmrB is identical to the F36V mutant of FKBP12
DmrC is identical to the T82L mutant of FRB

More Information

Source

DmrA and DmrB monoclonal antibodies: mouse
DmrC polyclonal antibody: rabbit

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

iDimerize antibodies

Notice to purchaser

Western blot detection of a DmrC-tagged protein with the DmrC Polyclonal Antibody (Cat. No. 635091)

The DmrC Polyclonal Antibody (Cat. No. 635091) can detect as little as 16 ng of DmrC protein

Notice to purchaser

Western blot detection of a DmrB-tagged protein using the DmrB Monoclonal Antibody (Cat.No. 635090)

635090: DmrB Monoclonal Antibody

Notice to purchaser

Western blot detection of a DmrA-tagged protein using the DmrA Monoclonal Antibody (Cat. No. 635089)

635089: DmrA Monoclonal Antibody

Overview

More Information

Source

Additional product information

Related links