Gel extraction with silica resin—NucleoTrap kits
The NucleoTrap DNA extraction kit allows you to extract DNA fragments from solution (desalination) or agarose gels. The kit can also be used for the removal of nucleotides and/or biotin after labeling reactions, as well as for the removal of enzymes.
The NucleoTrap DNA extraction kit allows you to extract DNA fragments from solution (desalination) or agarose gels. The kit can also be used for the removal of nucleotides and/or biotin after labeling reactions, as well as for the removal of enzymes.
A specially activated spherical silica matrix makes it possible to extract DNA fragments from agarose gels or solvents in a very mild manner, without the use of organic solvents. Nucleic acids are bound to the NucleoTrap matrix in the presence of a chaotropic salt. The hydrate shell of the nucleic acids is destroyed by this binding buffer, thus facilitating adsorption to the specially treated NucleoTrap matrix. A washing step is followed by the elution of DNA with water or TE buffer.
The NucleoTrap gel extraction kit is designed to extract fragments from agarose gels or from aqueous solutions in sizes ranging from 20 bp to 20 kb. Each 10 µl of suspension can extract 2.5 µg of DNA.
Overview
- High binding capacity even for very small fragments (>20 bp)
- Large DNA fragments are not sheared and/or degraded
More Information
| Technology | Silica matrix |
| Format | Silica bead suspension |
| Starting material | 20–600 mg agarose gel |
| Fragment size | 20 bp–20 kb |
| Typical recovery | 50–90% |
| A260/280 | 1.7–1.9 |
| Elution volume | 20–50 µl |
| Preparation time | 60 min/6 preps |
| Binding capacity | 6 µg/10 µl suspension |
Applications
Purified DNA is suitable for:
- Cloning
- Sequencing
- PCR
- Restriction analysis
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
DNA cleanup: two applications in one kit
The NucleoSpin Gel and PCR Clean-Up procedure is the easiest way to purify DNA fragments from agarose gels or PCR reactions. The kit includes one buffer for both applications, which contains a pH indicator that displays the correct pH for optimal kit performance. The purification procedure for samples from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities.
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