Soil DNA isolation—NucleoSpin Soil
NucleoSpin Soil for the isolation of total DNA from soil microorganisms includes two alternative lysis buffers and an additional Enhancer SX, which can be combined with both lysis buffers. The resulting four different lysis systems make the kit suitable for all types of different samples, which is important because soil is very heterogeneous in pH and the amounts of organic or inorganic matter, such as humic substances or polysaccharides.
NucleoSpin Soil for the isolation of total DNA from soil microorganisms includes two alternative lysis buffers and an additional Enhancer SX, which can be combined with both lysis buffers. The resulting four different lysis systems make the kit suitable for all types of different samples, which is important because soil is very heterogeneous in pH and the amounts of organic or inorganic matter, such as humic substances or polysaccharides. The silica membranes in NucleoSpin Soil Columns are optimized for quantitative DNA binding. MN bead tubes, containing strong ceramic beads, allow efficient lysis of microorganisms in the sample. NucleoSpin Inhibitor Removal Columns are included for convenient elimination of contaminants.
NucleoSpin Soil can process up to 500 mg (wet weight) of starting material. Depending on the individual sample, typical yields are in the range of 2 to 10 µg of DNA. The eluted DNA is ready to use (undiluted) for subsequent reactions such as PCR, restriction analysis, etc.
Overview
- Extract DNA from soil, sludge, and sediment
- Four lysis options accommodate a variety of samples
- Designed for isolation of DNA from Gram-positive and Gram-negative bacteria, archaea, fungi, and algae present in soil, sludge, or sediment
- Kit includes ready-to-use bead tubes with ceramic beads
- Isolated DNA is free of PCR inhibitors
More Information
| Technology | Silica membrane |
| Format | Mini spin columns |
| Starting material | <500 mg soil, sludge, or sediment |
| Typical yield | 2–10 µg |
| Elution volume | 30–100 µl |
| Preparation time | <90 min (10 preps) |
| Binding capacity | 50 µg |
Applications
- Extract total DNA from microorganisms in soil and sediment, and free DNA in soil
- Quantify and detect microorganisms from environmental samples
- DNA is suitable for Southern blotting, array technology, RT-PCR, and more
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Are you extracting DNA from microbes in soil, sludge, or sediment?
We know that this can be challenging, since soil samples vary widely in their pH and the amount and composition of organic and inorganic matter, including PCR inhibitors, polysaccharides, and humic substances. The NucleoSpin Soil DNA isolation kit removes these contaminants, so you can easily detect and quantify microorganisms including Gram-positive and Gram-negative bacteria, archaea, yeast, fungi, and algae.
See the data Other gDNA extraction kitsTakara Bio USA, Inc.
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